Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenyl-5'-yl-imidodiphosphate | maximal activation of CDP reduction at 4 mM | Mus musculus | |
ATP | reduction of CDP is dependent on ATP or adenyl-5'-yl iminodiphosphate | Mus musculus | |
dihydrolipoic acid | slight stimulation | Mus musculus | |
dithiothreitol | slight activation | Mus musculus | |
dTTP | stimulation of GDP reduction | Mus musculus | |
EDTA | reversible stimulation of GDP reduction, irreversible inhibition of CDP reduction | Mus musculus | |
additional information | enzyme of cells first treated with 2,6-dichlorophenolindophenol has a complete dependence on NADPH which can also be met by dithiothreitol or dihydrolipoic acid | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | 4 mM, 50% inhibition of GDP reduction in the presence of dTTP | Mus musculus | |
bathophenanthroline sulfonate | 5 mM, almost complete inhibition of CDP and GDP reduction | Mus musculus | |
dATP | 0.0033 mM, 50% inhibition of CDP reduction, 0.0036 mM, 50% inhibition of GDP reduction; inhibition of CDP reduction | Mus musculus | |
dCTP | 1 mM, 50% inhibition of CDP reduction | Mus musculus | |
dGTP | 0.08 mM, 50% inhibition of CDP reduction, 0.19 mM, 50% inhibition of GDP reduction; inhibition of CDP reduction | Mus musculus | |
dTTP | 0.2 mM, 50% inhibition of CDP reduction | Mus musculus | |
EDTA | reversible stimulation of GDP reduction, irreversible inhibition of CDP reduction | Mus musculus | |
Hydroxyurea | 1 mM, 98 and 81% inhibition of CDP and GDP reduction respectively | Mus musculus | |
Mg2+ | 1-5 mM, 10-20% inhibition of GDP reduction; inhibition of CDP reduction | Mus musculus | |
sodium arsenite | 0.025 mM, almost complete inhibition of CDP reduction, 86% inhibition of GDP reduction | Mus musculus | |
Thenoyltrifluoroacetone | 5 mM, almost complete inhibition of CDP and GDP reduction | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
CDP | - |
Mus musculus | |
0.24 | - |
GDP | - |
Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Novikoff ascites tumor cells, partial | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + reduced thioredoxin | - |
Mus musculus | 2'-deoxy-ADP + oxidized thioredoxin + H2O | - |
ir | |
CDP + reduced thioredoxin | - |
Mus musculus | 2'-deoxy-CDP + oxidized thioredoxin + H2O | - |
ir | |
GDP + reduced thioredoxin | - |
Mus musculus | 2'-deoxy-GDP + oxidized thioredoxin + H2O | - |
ir | |
ribonucleoside diphosphate + reduced thioredoxin | - |
Mus musculus | 2'-deoxyribonucleoside diphosphate + oxidized thioredoxin + H2O | - |
ir | |
UDP + reduced thioredoxin | - |
Mus musculus | 2'-deoxy-UDP + oxidized thioredoxin + H2O | - |
ir |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | slight stimulation | Mus musculus |