General Stability | Organism |
---|---|
significant loss of activity during the purification procedure, lyophilization results in a complete loss of activity | Neobacillus niacini |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycerol | at a final concentration 10% (v/v), inactivates to 30% of the initial activity | Neobacillus niacini | |
KCN | - |
Neobacillus niacini | |
additional information | not inhibited by NaCl | Neobacillus niacini | |
Na2S | partially purified 6-HDH, 90% loss of activity | Neobacillus niacini |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
6-Hydroxynicotinate | pH 7.5, 30°C | Neobacillus niacini |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Neobacillus niacini | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains iron, iron-sulfur centres | Neobacillus niacini | |
sulfur | contains acid-labile sulfur, iron-sulfur centres | Neobacillus niacini |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
34000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
85000 | - |
x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
100000 | 140000 | non-denaturing PAGE, gel filtration | Neobacillus niacini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Neobacillus niacini | acts together with nicotinate dehydrogenase in nicotinate catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neobacillus niacini | - |
- |
- |
Neobacillus niacini | - |
strain DSM 2923 | - |
Purification (Comment) | Organism |
---|---|
- |
Neobacillus niacini |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
6-hydroxynicotinate + H2O + O2 = 2,6-dihydroxynicotinate + H2O2 | contains [2Fe-2S] iron-sulfur centres, FAD and molybdenum, it also has a catalytically essential, labile selenium that can be removed by reaction with cyanide, in Bacillus niacini, this enzyme is required for growth on nicotinic acid | Neobacillus niacini |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
pH 7.5, 30°C | Neobacillus niacini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxynicotinate + H2O + 2,6-dichlorophenol indophenol | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced 2,6-dichlorophenol indophenol | - |
? | |
6-hydroxynicotinate + H2O + methylene blue | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 40% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced methylene blue | - |
? | |
6-hydroxynicotinate + H2O + nitroblue tetrazolium chloride | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced nitroblue tetrazolium chloride | - |
? | |
6-hydroxynicotinate + H2O + O2 | - |
Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 | - |
? | |
6-hydroxynicotinate + H2O + O2 | highly specific for 6-hydroxynicotinate | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 | - |
? | |
6-hydroxynicotinate + H2O + phenazine ethosulfate | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol is a good electron acceptor | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced phenazine ethosulfate | - |
? | |
6-hydroxynicotinate + H2O + thiazolyl blue | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, same activity as with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced thiazolyl blue | - |
? | |
6-hydroxynicotinate + H2O + thionin | highly specific for 6-hydroxynicotinate, transfers electrons only to artificial acceptors of high redox potential, 2% of the activity with the mixture of phenazine ethosulfate and 2,6-dichlorophenol indophenol | Neobacillus niacini | 2,6-dihydroxynicotinate + H2O2 + reduced thionin | - |
? | |
additional information | no electron acceptors: Brilliant cresyl blue, cytochrome c, FAD, ferricyanide, FMN, menadione, NAD+, NADP+, and both viologens | Neobacillus niacini | ? | - |
? | |
additional information | acts together with nicotinate dehydrogenase in nicotinate catabolism | Neobacillus niacini | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 85000 + x * 34000 + x * 15000, SDS-PAGE | Neobacillus niacini |
Synonyms | Comment | Organism |
---|---|---|
6-HDH | - |
Neobacillus niacini |
6-hydroxynicotinate hydroxylase | - |
Neobacillus niacini |
6-hydroxynicotinic acid dehydrogenase | - |
Neobacillus niacini |
6-hydroxynicotinic acid hydroxylase | - |
Neobacillus niacini |
dehydrogenase, 6-hydroxynicotinate | - |
Neobacillus niacini |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neobacillus niacini |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Neobacillus niacini |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains FAD | Neobacillus niacini | |
iron-sulfur centre | contains iron-sulfur centres, iron and acid-labile sulfur | Neobacillus niacini | |
molybdenum cofactor | contains molybdenum | Neobacillus niacini |