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Literature summary for 1.17.2.2 extracted from
- Presence and role of a second disulphide bond in recombinant lupanine hydroxylase using site-directed mutagenesis with 143Cys/Ser and 124,143Cys/Ser mutations in Escherichia coli (2012), FEMS Microbiol. Lett., 334, 35-43.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudomonas sp. |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C124S/C143S | mutant displays no activity compared to wild-type | Pseudomonas sp. |
| C143S | mutant displays only 25% activity compared to wild-type | Pseudomonas sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | dithiothreitol-induced reduction of LH followed by Cd2+ treatment results in significant loss of activity in a dose-dependent manner | Pseudomonas sp. |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas sp. | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lupanine hydroxylase | - |
Pseudomonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudomonas sp. |
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Release 2023.1 (January 2023)
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