KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
lupanine | 25°C, pH 8.5 | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
ultracentrifugation studies | Pseudomonas sp. |
72000 | - |
1 * 72000, SDS-PAGE | Pseudomonas sp. |
74000 | - |
gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lupanine + pyrroloquinoline quinone + H2O | Pseudomonas sp. | lupanine is a quinolizidine alkaloid and is particularly abundant in species of the genus Lupinus. It can be degraded by bacteria. The first step in its catabolism by a Pseudomonas sp. is hydroxylation to 17-hydroxylupanine | 17-hydroxylupanine + pyrroloquinoline quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
69.7 | - |
25°C, pH 8.5 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lupanine + pyrroloquinoline quinone + H2O | - |
Pseudomonas sp. | 17-hydroxylupanine + pyrroloquinoline quinol | - |
? | |
lupanine + pyrroloquinoline quinone + H2O | lupanine is a quinolizidine alkaloid and is particularly abundant in species of the genus Lupinus. It can be degraded by bacteria. The first step in its catabolism by a Pseudomonas sp. is hydroxylation to 17-hydroxylupanine | Pseudomonas sp. | 17-hydroxylupanine + pyrroloquinoline quinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.3 | - |
lupanine | 25°C, pH 8.5 | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | enzyme is assayed over a range of pH from 7 to 10 in sodium/potassium phosphate, Tris/HCI and glycine/NaOH buffers | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | contains one heme group per molecule of enzyme | Pseudomonas sp. | |
pyrroloquinoline quinone | contains one molecule of pyrroloquinoline quinone per molecule of enzyme | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing, pH 3-10, reduced enzyme | - |
5 |
Pseudomonas sp. | isoelectric focusing, pH 3-10, oxidized enzyme | - |
5.3 |