Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADH | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.022 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.026 | - |
NADH | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
0.039 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27622 | - |
4 * 27622, calculated from amino acid sequence | Staphylococcus aureus |
27622 | - |
4 * 27622, ESI-TOF mass spectrometry | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus MRSA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography and Superose 12 gel filtration | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
at pH 8.0 and 30°C, the crude recombinant enzyme and enzyme after 2.9fold purification show specific activities of 5.51 and 16 units/mg, respectively | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Staphylococcus aureus | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Staphylococcus aureus MRSA | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Staphylococcus aureus | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Staphylococcus aureus MRSA | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 27622, calculated from amino acid sequence | Staphylococcus aureus |
homotetramer | 4 * 27622, ESI-TOF mass spectrometry | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
DapB | - |
Staphylococcus aureus |
DHDPR | - |
Staphylococcus aureus |
dihydrodipicolinate reductase | - |
Staphylococcus aureus |
EC 1.3.1.26 | formerly | Staphylococcus aureus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
both the native and His-tagged recombinant enzymes show an apparent melting temperature of approximately 65°C | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
NADH | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
20 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | native enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
21 | - |
NADH | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus | |
21 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | recombinant enzyme, at pH 8.0 and 30°C | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Staphylococcus aureus | |
NADPH | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the second step of the lysine biosynthesis pathway | Staphylococcus aureus |