Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADPH | in 50 mM Tris-HCl, pH 8.0, at 37°C | Pseudomonas aeruginosa | |
0.065 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate | in 50 mM Tris-HCl, pH 8.0, at 37°C | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
4 * 28000, calculated from amino acid sequence | Pseudomonas aeruginosa |
30000 | - |
4 * 30000, His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
120000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+ | - |
Pseudomonas aeruginosa | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O | - |
ir | |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+ | - |
Pseudomonas aeruginosa | (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 28000, calculated from amino acid sequence | Pseudomonas aeruginosa |
homotetramer | 4 * 30000, His-tagged enzyme, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
DapB | - |
Pseudomonas aeruginosa |
DHDPR | - |
Pseudomonas aeruginosa |
dihydrodipicolinate reductase | - |
Pseudomonas aeruginosa |
EC 1.3.1.26 | formerly | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | at 30°C and 50°C, approximately 57% and 85% of the enzymatic activity is retained. Between 50 and 60°C a fast decrease in activity is observed with increasing temperature. Complete loss of activity occurs above 60°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | at pH 7.0 and 9.0, approyimately 75% and 90% of the enzymatic activity is retained. Below pH 7.0 and above pH 9.0 a significant drop in activity is observed. At pH 11.0 an almost complete loss of activity is observed | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |