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Literature summary for 1.17.1.8 extracted from

  • Pearce, F.G.; Sprissler, C.; Gerrard, J.A.
    Characterization of dihydrodipicolinate reductase from Thermotoga maritima reveals evolution of substrate binding kinetics (2008), J. Biochem., 143, 617-623.
    View publication on PubMed
Search for more literature for 1.17.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL-1 Blue cells Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
2,3-dihydrodipicolinate the enzyme is inhibited by high concentrations of 2,3-dihydrodipicolinate Thermotoga maritima
NADH
-
 Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
 NADPH apparent value, at 30°C Thermotoga maritima
0.0018
-
 NADH apparent value, at 45°C Thermotoga maritima
0.0021
-
 NADPH apparent value, at 45°C Thermotoga maritima
0.0025
-
 NADH apparent value, at 30°C Thermotoga maritima
0.0076
-
 2,3-dihydrodipicolinate apparent value, at 30°C Thermotoga maritima
0.013
-
 2,3-dihydrodipicolinate apparent value, at 45°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X1K8
-
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 Q9X1K8
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydrodipicolinate + NAD(P)H
-
 Thermotoga maritima 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?
2,3-dihydrodipicolinate + NAD(P)H
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 2,3,4,5-tetrahydrodipicolinate + NAD(P)+
-
 ?
2,3-dihydrodipicolinate + NADH + H+
-
 Thermotoga maritima 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O
-
 r
2,3-dihydrodipicolinate + NADH + H+
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O
-
 r

Subunits

Subunits Comment Organism
tetramer x-ray crystallography Thermotoga maritima

Synonyms

Synonyms Comment Organism
DHDPR
-
 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80 95 the enzyme is stable for up to 48 h at 80°C, the melting temperature of DHDPR is at 95.7°C Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.093
-
 NADH apparent value, at 30°C Thermotoga maritima
0.49
-
 NADH apparent value, at 45°C Thermotoga maritima
2.6
-
 NADPH apparent value, at 30°C Thermotoga maritima
8.1
-
 2,3-dihydrodipicolinate apparent value, at 30°C Thermotoga maritima
17
-
 NADPH apparent value, at 45°C Thermotoga maritima
19
-
 2,3-dihydrodipicolinate apparent value, at 45°C Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme has a greater affinity for NADPH than for NADH Thermotoga maritima
NADPH the enzyme has a greater affinity for NADPH than for NADH Thermotoga maritima

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0051
-
 NADH at 30°C Thermotoga maritima
0.0081
-
 NADH at 45°C Thermotoga maritima