Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1 Blue cells | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-dihydrodipicolinate | the enzyme is inhibited by high concentrations of 2,3-dihydrodipicolinate | Thermotoga maritima | |
NADH | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
NADPH | apparent value, at 30°C | Thermotoga maritima | |
0.0018 | - |
NADH | apparent value, at 45°C | Thermotoga maritima | |
0.0021 | - |
NADPH | apparent value, at 45°C | Thermotoga maritima | |
0.0025 | - |
NADH | apparent value, at 30°C | Thermotoga maritima | |
0.0076 | - |
2,3-dihydrodipicolinate | apparent value, at 30°C | Thermotoga maritima | |
0.013 | - |
2,3-dihydrodipicolinate | apparent value, at 45°C | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X1K8 | - |
- |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | Q9X1K8 | - |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydrodipicolinate + NAD(P)H | - |
Thermotoga maritima | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NAD(P)H | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NADH + H+ | - |
Thermotoga maritima | 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O | - |
r | |
2,3-dihydrodipicolinate + NADH + H+ | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | 2,3,4,5-tetrahydrodipicolinate + NAD+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | x-ray crystallography | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
DHDPR | - |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 95 | the enzyme is stable for up to 48 h at 80°C, the melting temperature of DHDPR is at 95.7°C | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.093 | - |
NADH | apparent value, at 30°C | Thermotoga maritima | |
0.49 | - |
NADH | apparent value, at 45°C | Thermotoga maritima | |
2.6 | - |
NADPH | apparent value, at 30°C | Thermotoga maritima | |
8.1 | - |
2,3-dihydrodipicolinate | apparent value, at 30°C | Thermotoga maritima | |
17 | - |
NADPH | apparent value, at 45°C | Thermotoga maritima | |
19 | - |
2,3-dihydrodipicolinate | apparent value, at 45°C | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzyme has a greater affinity for NADPH than for NADH | Thermotoga maritima | |
NADPH | the enzyme has a greater affinity for NADPH than for NADH | Thermotoga maritima |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
NADH | at 30°C | Thermotoga maritima | |
0.0081 | - |
NADH | at 45°C | Thermotoga maritima |