Application | Comment | Organism |
---|---|---|
medicine | design and evaluation of dihydropicolinate reductase inhibitors as broad spectrum antibiotics and herbicides | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
dapB gene, overexpression from plasmid in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,6-pyridinedicarboxylic acid | dead-end inhibitor | Escherichia coli | |
NADP+ | product inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
NADH | - |
Escherichia coli | |
0.0021 | - |
thio-NADPH | - |
Escherichia coli | |
0.004 | - |
reduced 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | |
0.0056 | - |
NADPH | - |
Escherichia coli | |
0.009 | - |
reduced nicotinamide hypoxanthine dinucleotide phosphate | - |
Escherichia coli | |
0.05 | - |
2,3-dihydrodipicolinate | - |
Escherichia coli | |
0.052 | - |
alpha-NADPH | - |
Escherichia coli | |
0.08 | - |
3'-NADPH | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydrodipicolinate + NAD(P)H | Escherichia coli | lysine and diaminopimelate biosynthesis pathway | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpression in Escherichia coli from plasmid | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | stereospecific transfer of the 4R hydrogen atom of the reduced nucleotide, as a hydrid ion, to the 4 position of dihydrodipicolinate | Escherichia coli | |
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H + H+ | kinetic and chemical mechanism | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
398 | - |
purified enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydrodipicolinate + 3'-NADPH + H+ | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + 3'-NADP+ + H2O | - |
r | |
2,3-dihydrodipicolinate + alpha-NADPH + H+ | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + alpha-NADP+ + H2O | - |
r | |
2,3-dihydrodipicolinate + NAD(P)H | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + NAD(P)H | lysine and diaminopimelate biosynthesis pathway | Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + NAD(P)+ | - |
? | |
2,3-dihydrodipicolinate + reduced 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + oxidized 3-acetylpyridine adenine dinucleotide | - |
? | |
2,3-dihydrodipicolinate + reduced 3-acetylpyridine adenine dinucleotide | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + oxidized 3-acetylpyridine adenine dinucleotide + H2O | - |
r | |
2,3-dihydrodipicolinate + reduced nicotinamide hypoxanthine dinucleotide phosphate | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + oxidized nicotinamide hypoxanthine dinucleotide phosphate + H2O | - |
r | |
2,3-dihydrodipicolinate + thio-NADPH + H+ | - |
Escherichia coli | 2,3,4,5-tetrahydrodipicolinate + thio-NADP+ + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 28000-28758, SDS-PAGE and electrospray mass spectrometry | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DHPR | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | stereospecific transfer of the 4R hydrogen atom of the reduced nucleotide, as a hydrid ion, to the 4 position of dihydrodipicolinate | Escherichia coli | |
NADH | twice as effective as NADPH | Escherichia coli | |
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
2,6-pyridinedicarboxylic acid | - |
Escherichia coli | |
24 | - |
NADP+ | - |
Escherichia coli |