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Literature summary for 1.17.1.5 extracted from

  • Nagel, M.; Andreesen, J.R.
    Molybdenum-dependent degradation of nicotinic acid by Bacillus sp. DSM 2923 (1989), FEMS Microbiol. Lett., 59, 147-152.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information 4 chlorate-resistant mutants are no longer able to degrade nicotinate Neobacillus niacini

Inhibitors

Inhibitors Comment Organism Structure
KCN 2 mM, complete loss of enzyme activity in anaerobically incubated crude extract Neobacillus niacini

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme activity increases with increasing concentrations of molybdate in growth medium Neobacillus niacini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinate + H2O + electron acceptor Neobacillus niacini nicotinate degradation 6-hydroxynicotinate + reduced electron acceptor
-
?
nicotinate + H2O + electron acceptor Neobacillus niacini DSM 2923 nicotinate degradation 6-hydroxynicotinate + reduced electron acceptor
-
?

Organism

Organism UniProt Comment Textmining
Neobacillus niacini
-
strain AS1 isolated from the culture obtained by DSM
-
Neobacillus niacini DSM 2923
-
strain AS1 isolated from the culture obtained by DSM
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2
-
crude extract, high activity achieved if nicotinate is the only substrate Neobacillus niacini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + H2O + electron acceptor high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
?
nicotinate + H2O + electron acceptor nicotinate degradation Neobacillus niacini 6-hydroxynicotinate + reduced electron acceptor
-
?
nicotinate + H2O + electron acceptor high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor Neobacillus niacini DSM 2923 6-hydroxynicotinate + reduced electron acceptor
-
?
nicotinate + H2O + electron acceptor nicotinate degradation Neobacillus niacini DSM 2923 6-hydroxynicotinate + reduced electron acceptor
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Neobacillus niacini

Cofactor

Cofactor Comment Organism Structure
molybdenum cofactor molybdoenzyme with eubacterial molybdenum cofactor Neobacillus niacini