Protein Variants | Comment | Organism |
---|---|---|
additional information | 4 chlorate-resistant mutants are no longer able to degrade nicotinate | Neobacillus niacini |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCN | 2 mM, complete loss of enzyme activity in anaerobically incubated crude extract | Neobacillus niacini |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity increases with increasing concentrations of molybdate in growth medium | Neobacillus niacini |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + electron acceptor | Neobacillus niacini | nicotinate degradation | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | Neobacillus niacini DSM 2923 | nicotinate degradation | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neobacillus niacini | - |
strain AS1 isolated from the culture obtained by DSM | - |
Neobacillus niacini DSM 2923 | - |
strain AS1 isolated from the culture obtained by DSM | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
crude extract, high activity achieved if nicotinate is the only substrate | Neobacillus niacini |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + H2O + electron acceptor | high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | nicotinate degradation | Neobacillus niacini | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | high substrate specificity, mixture of phenazine ethosulfate and 2,6-dichlorophenolindophenol as electron acceptor | Neobacillus niacini DSM 2923 | 6-hydroxynicotinate + reduced electron acceptor | - |
? | |
nicotinate + H2O + electron acceptor | nicotinate degradation | Neobacillus niacini DSM 2923 | 6-hydroxynicotinate + reduced electron acceptor | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Neobacillus niacini |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
molybdenum cofactor | molybdoenzyme with eubacterial molybdenum cofactor | Neobacillus niacini |