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Literature summary for 1.17.1.4 extracted from

  • Sakai, T.; Jun, H.K.
    Purification, crystallization, and some properties of xanthine dehydrogenase from Pseudomonas synxantha A 3 (1979), Agric. Biol. Chem., 43, 753-760.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuriphenyl sulfonic acid 98% inhibition at 0.001 mM Pseudomonas synxantha
Ag2+ complete inhibition of hypoxanthine oxidation at 0.01 mM Pseudomonas synxantha
Cu2+ 14% inhibition of hypoxanthine oxidation at 0.1 mM Pseudomonas synxantha
Hg2+ complete inhibition of hypoxanthine oxidation at 0.1 mM Pseudomonas synxantha
KCN 37% inhibition at 1 mM Pseudomonas synxantha

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000 + x * 76000, SDS-PAGE Pseudomonas synxantha
76000
-
x * 54000 + x * 76000, SDS-PAGE Pseudomonas synxantha
540000
-
gel filtration Pseudomonas synxantha

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H2O Pseudomonas synxantha preferred substrate xanthine + NADH + H+
-
?
purine + NAD+ + H2O Pseudomonas synxantha poor substrate ? + NADH
-
?
xanthine + NAD+ + H2O Pseudomonas synxantha 75% of the activity compared to hypoxanthine urate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas synxantha
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to crystalline state, chromatography techniques Pseudomonas synxantha

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Pseudomonas synxantha
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
-
Pseudomonas synxantha

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dithiopurine + NAD+ + H2O 26% of the activity compared to hypoxanthine Pseudomonas synxantha ? + NADH
-
?
2-thioxanthine + NAD+ + H2O 57% of the activity compared to hypoxanthine Pseudomonas synxantha 2-thiourate + NADH
-
?
6-thioxanthine + NAD+ + H2O 63% of the activity compared to hypoxanthine Pseudomonas synxantha 6-thiourate + NADH
-
?
8-azahypoxanthine + NAD+ + H2O 42% of the activity compared to hypoxanthine Pseudomonas synxantha 8-azaxanthine + NADH
-
?
adenine-N1-oxide + NAD+ + H2O 3.4% of the activity compared to hypoxanthine Pseudomonas synxantha ? + NADH
-
?
hypoxanthine + 2,6-dichlorophenolindophenol + H2O considerable activity Pseudomonas synxantha xanthine + ?
-
?
hypoxanthine + NAD+ + H2O preferred substrate Pseudomonas synxantha xanthine + NADH + H+
-
?
hypoxanthine + phenazine methosulfate + H2O low activity Pseudomonas synxantha urate + ?
-
?
isoguanine + NAD+ + H2O 7.3% of the activity compared to hypoxanthine Pseudomonas synxantha ? + NADH
-
?
purine + NAD+ + H2O poor substrate Pseudomonas synxantha ? + NADH
-
?
xanthine + NAD+ + H2O 75% of the activity compared to hypoxanthine Pseudomonas synxantha urate + NADH
-
?

Subunits

Subunits Comment Organism
? x * 54000 + x * 76000, SDS-PAGE Pseudomonas synxantha

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pseudomonas synxantha

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable for 10 min, pH 8.5 Pseudomonas synxantha

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.8
-
Pseudomonas synxantha

Cofactor

Cofactor Comment Organism Structure
FAD
-
Pseudomonas synxantha
NAD+ cannot be replaced by NADP+ Pseudomonas synxantha