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Literature summary for 1.17.1.4 extracted from

  • Waud, W.R.; Rajagopalan, K.V.
    The mechanism of conversion of rat liver xanthine dehydrogenase from an NAD+-dependent form (type D) to an O2-dependent form (type O) (1976), Arch. Biochem. Biophys., 172, 365-379.
    View publication on PubMed
Search for more literature for 1.17.1.4

Activating Compound

Activating Compound Comment Organism Structure
DTT recovery from oxidase to dehydrogenase type Bos taurus
DTT recovery from oxidase to dehydrogenase type Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
5,5-dithiobis-(2-nitrobenzoate) conversion of dehydrogenase type D to oxidase type O due to modification of a limited number of critical sulfhydryl groups Rattus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mo
-
 Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 x * 150000, SDS-urea -PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + NAD+ + H2O Bos taurus NAD+-dependent dehydrogenase type D urate + NADH
-
 ?
xanthine + NAD+ + H2O Rattus sp. NAD+-dependent dehydrogenase type D urate + NADH
-
 ?
xanthine + NAD+ + O2 + H2O + H+ Rattus sp. heat-treated intermediate dehydrogenase/oxidase type O urate + NADH + H2O2
-
 ?
xanthine + O2 + H2O Bos taurus
-
 urate + O2- + 2 H+
-
 ?
xanthine + O2 + H2O Rattus sp. NAD+-independent trypsin-treated oxidase type O urate + O2- + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified as antibody complex Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus sp.
-
milk
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + electron acceptor + H2O conversion to the oxidase type O by trypsinization leads to 80-100% decrease in the oxidation rate of NADH, conversion to the oxidase type O by heat-treatment leads to a diminution of NADH oxidation Rattus sp. NAD+ + reduced electron acceptor
-
 ?
xanthine + 2,6-dichlorophenolindophenol + H2O
-
 Bos taurus urate + ?
-
 ?
xanthine + 2,6-dichlorophenolindophenol + H2O greatly enhanced activity for dehydrogenase type D and trypsin- or heat-treated oxidase types O Rattus sp. urate + ?
-
 ?
xanthine + cytochrome c + H2O enhanced activity for heat- and trypsin-treated oxidase types O Rattus sp. urate + reduced cytochrome c
-
 ?
xanthine + ferricyanide + H2O low activity for both dehydrogenase type D and oxidase type O Rattus sp. urate + ferrocyanide
-
 ?
xanthine + methylene blue + H2O enhanced oxidation of xanthine for dehydrogenase type D and trypsin- or heat-treated oxidase type O Rattus sp. urate + reduced methylene blue
-
 ?
xanthine + NAD+ + H2O NAD+-dependent dehydrogenase type D Bos taurus urate + NADH
-
 ?
xanthine + NAD+ + H2O NAD+-dependent dehydrogenase type D Rattus sp. urate + NADH
-
 ?
xanthine + NAD+ + O2 + H2O + H+ heat-treated intermediate dehydrogenase/oxidase type O Rattus sp. urate + NADH + H2O2
-
 ?
xanthine + O2 + H2O
-
 Bos taurus urate + O2- + 2 H+
-
 ?
xanthine + O2 + H2O NAD+-independent trypsin-treated oxidase type O Rattus sp. urate + O2- + 2 H+
-
 ?

Subunits

Subunits Comment Organism
? x * 150000, SDS-urea -PAGE Rattus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 conversion to an intermediate dehydrogenase-oxidase type O form in 30 min Rattus sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Bos taurus
FAD trypsin-treated oxidase type O shows absence of FAD, heat-treated oxidase type O shows small measurable FAD Rattus sp.
NAD+
-
 Bos taurus
NAD+
-
 Rattus sp.