Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxypyrazolo(3,4-d)pyrimidine | i.e. allopurinol; rapid inactivation under anaerobic conditions at 0.1 mM | Delftia acidovorans | |
KCN | irreversible inactivation | Delftia acidovorans | |
Sodium dithionite | reduction of enzyme | Delftia acidovorans | |
xanthine | reduction of enzyme | Delftia acidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
xanthine | electron acceptor: NAD+ | Delftia acidovorans | |
0.16 | - |
NAD+ | electron donor: xanthine | Delftia acidovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron-sulfur-center | 2 Fe2-S2-clusters | Delftia acidovorans | |
molybdenum-center | contains molybdopterin mononucleotide rather than molybdopterin dinucleotide | Delftia acidovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE | Delftia acidovorans |
90000 | - |
alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE | Delftia acidovorans |
287000 | - |
gel filtration | Delftia acidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Delftia acidovorans | - |
urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | 3.6 mol per alpha/beta-protomer | Delftia acidovorans |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Delftia acidovorans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Delftia acidovorans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Delftia acidovorans |
Storage Stability | Organism |
---|---|
-80°C, 6 months | Delftia acidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + electron acceptor + H2O | NADH diaphorase activity with several acceptors | Delftia acidovorans | NAD+ + reduced electron acceptor | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | - |
Delftia acidovorans | urate + ? | - |
? | |
xanthine + ferricyanide + H2O | - |
Delftia acidovorans | urate + ferrocyanide | - |
? | |
xanthine + NAD+ + H2O | - |
Delftia acidovorans | urate + NADH | - |
? | |
xanthine + nitroblue tetrazolium + H2O | - |
Delftia acidovorans | urate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | alpha1, x * 90000 + beta1, x * 60000, SDS-PAGE | Delftia acidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
120 | - |
xanthine | electron acceptor: NAD+ | Delftia acidovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Delftia acidovorans | |
additional information | molybdoironflavoprotein: ratio of iron to FAD to molybdenum is 4 : 1 : 1 | Delftia acidovorans | |
NAD+ | physiological cofactor | Delftia acidovorans |