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Literature summary for 1.17.1.4 extracted from

  • Waud, W.R.; Rajagopalan, K.V.
    Purification and properties of the NAD+-dependent (type D) and O2-dependent (type O) forms of rat liver xanthine dehydrogenase (1976), Arch. Biochem. Biophys., 172, 354-364.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methylene blue 6fold activation of aerobic rate of oxidation of xanthine Rattus sp.
NAD+ 6fold stimulation of aerobic rate of oxidation of xanthine Rattus sp.

General Stability

General Stability Organism
treatment accelerating conversion of dehydrogenase type D to oxidase type O is lyophilization, storage at -20°C, exposure to 20% ammonium sulfate, 0.5 mM NAD+ or 0.01 mM xanthine Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
Superoxide dismutase complete inhibition of the xanthine-cytochrome c activity for oxidase type O, lesser inhibition for dehydrogenase type D Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0017
-
xanthine dehydrogenase type D, absence of NAD+ Rattus sp.
0.002
-
xanthine oxidase type O, absence of NAD+ Rattus sp.
0.0026
-
xanthine cosubstrate NAD+ Rattus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe
-
Rattus sp.
Iron-sulfur-center
-
Rattus sp.
Mo
-
Rattus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
x * 150000, SDS-urea -PAGE Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xanthine + NAD+ + H2O Rattus sp. NAD+-dependent dehydrogenase type D urate + NADH
-
?
xanthine + NAD+ + O2 + H2O + H+ Rattus sp.
-
urate + NADH + H2O2
-
?
xanthine + O2 + H2O Rattus sp.
-
urate + O2- + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nearly homogenous, dehydrogenase type D, chromatography steps, oxidase type O purified by nearly the same procedure, but heated to 60°C after homogenization Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + electron acceptor + H2O only dehydrogenase type D shows considerable activities, not oxidase type O Rattus sp. NAD+ + reduced electron acceptor
-
?
xanthine + cytochrome c + H2O
-
Rattus sp. urate + reduced cytochrome c
-
?
xanthine + ferricyanide + H2O low activity for both dehydrogenase type D and oxidase type O Rattus sp. urate + ferrocyanide
-
?
xanthine + methylene blue + H2O same activity for dehydrogenase type D and oxidase type O Rattus sp. urate + reduced methylene blue
-
?
xanthine + NAD+ + H2O NAD+-dependent dehydrogenase type D Rattus sp. urate + NADH
-
?
xanthine + NAD+ + O2 + H2O + H+
-
Rattus sp. urate + NADH + H2O2
-
?
xanthine + O2 + H2O
-
Rattus sp. urate + O2- + 2 H+
-
?

Subunits

Subunits Comment Organism
? x * 150000, SDS-urea -PAGE Rattus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
conversion of dehydrogenase type D to oxidase type O Rattus sp.

Cofactor

Cofactor Comment Organism Structure
FAD two FAD per enzyme molecule Rattus sp.
additional information molybdoironflavoprotein: ratio of 2 : 1.4 : 7.6 of FAD to molybdenum to Fe-S Rattus sp.
NAD+ cannot be replaced by NADP+ Rattus sp.