Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methylene blue | 6fold activation of aerobic rate of oxidation of xanthine | Rattus sp. | |
NAD+ | 6fold stimulation of aerobic rate of oxidation of xanthine | Rattus sp. |
General Stability | Organism |
---|---|
treatment accelerating conversion of dehydrogenase type D to oxidase type O is lyophilization, storage at -20°C, exposure to 20% ammonium sulfate, 0.5 mM NAD+ or 0.01 mM xanthine | Rattus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Superoxide dismutase | complete inhibition of the xanthine-cytochrome c activity for oxidase type O, lesser inhibition for dehydrogenase type D | Rattus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
xanthine | dehydrogenase type D, absence of NAD+ | Rattus sp. | |
0.002 | - |
xanthine | oxidase type O, absence of NAD+ | Rattus sp. | |
0.0026 | - |
xanthine | cosubstrate NAD+ | Rattus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Rattus sp. | |
Iron-sulfur-center | - |
Rattus sp. | |
Mo | - |
Rattus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
x * 150000, SDS-urea -PAGE | Rattus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Rattus sp. | NAD+-dependent dehydrogenase type D | urate + NADH | - |
? | |
xanthine + NAD+ + O2 + H2O + H+ | Rattus sp. | - |
urate + NADH + H2O2 | - |
? | |
xanthine + O2 + H2O | Rattus sp. | - |
urate + O2- + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nearly homogenous, dehydrogenase type D, chromatography steps, oxidase type O purified by nearly the same procedure, but heated to 60°C after homogenization | Rattus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + electron acceptor + H2O | only dehydrogenase type D shows considerable activities, not oxidase type O | Rattus sp. | NAD+ + reduced electron acceptor | - |
? | |
xanthine + cytochrome c + H2O | - |
Rattus sp. | urate + reduced cytochrome c | - |
? | |
xanthine + ferricyanide + H2O | low activity for both dehydrogenase type D and oxidase type O | Rattus sp. | urate + ferrocyanide | - |
? | |
xanthine + methylene blue + H2O | same activity for dehydrogenase type D and oxidase type O | Rattus sp. | urate + reduced methylene blue | - |
? | |
xanthine + NAD+ + H2O | NAD+-dependent dehydrogenase type D | Rattus sp. | urate + NADH | - |
? | |
xanthine + NAD+ + O2 + H2O + H+ | - |
Rattus sp. | urate + NADH + H2O2 | - |
? | |
xanthine + O2 + H2O | - |
Rattus sp. | urate + O2- + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 150000, SDS-urea -PAGE | Rattus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
conversion of dehydrogenase type D to oxidase type O | Rattus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | two FAD per enzyme molecule | Rattus sp. | |
additional information | molybdoironflavoprotein: ratio of 2 : 1.4 : 7.6 of FAD to molybdenum to Fe-S | Rattus sp. | |
NAD+ | cannot be replaced by NADP+ | Rattus sp. |