Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ammeline | competitive inhibition of xanthine oxidation Ki: 0.083 mM, uncompetitive inhibition of NADH oxidation Ki: 0.063 mM | Gallus gallus | |
arsenite | gradual inhibition of xanthine-2,6-dichloroindophenol-activity, paralleled by a corresponding increase of NADH-2,6-dichloroindophenol-activity | Gallus gallus | |
KCN | complete inhibition of xanthine dehydrogenase activity, 70% reduction of diaphorase activity | Gallus gallus | |
methanol | slight inhibition of NAD+ reduction at 1.5 M, rapid inactivation if NAD+ is replaced by 2,6-dichloroindophenol, enhanced NADH diaphorase activity | Gallus gallus | |
NAD+ | competitive inhibition of NADH oxidation, Ki: 0.0143 mM | Gallus gallus | |
Urea | competitive inhibition of xanthine oxidation Ki: 0.28 M, uncompetitive inhibition of NADH oxidation Ki: 1 M | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | - |
Gallus gallus | |
Mo | - |
Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
sedimentation equilibrium measurements | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xanthine + NAD+ + H2O | Gallus gallus | ping-pong reaction mechanism | urate + NADH | - |
r | |
xanthine + O2 + H2O | Gallus gallus | low activity | urate + O2- + 2 H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
chicken | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + electron acceptor + H2O | NADH diaphorase activity with several acceptors | Gallus gallus | NAD+ + reduced electron acceptor | - |
? | |
NADH + electron acceptor + H2O | 2,6-dichloroindophenol, 3-acetylpyridine-adenine dinucleotide, methylene blue, phenazine methosulfate or trinitrobenzene sulfonate | Gallus gallus | NAD+ + reduced electron acceptor | - |
? | |
xanthine + 2,6-dichlorophenolindophenol + H2O | same activity compared to NAD+ as electron acceptor | Gallus gallus | urate + ? | - |
r | |
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O | same activity compared to NAD+ | Gallus gallus | urate + 3-acetylpyridine-adenine dinucleotide(H) | - |
r | |
xanthine + 3-acetylpyridine-adenine dinucleotide+ + H2O | low reverse activity | Gallus gallus | urate + 3-acetylpyridine-adenine dinucleotide(H) | - |
r | |
xanthine + methylene blue + H2O | - |
Gallus gallus | urate + reduced methylene blue | - |
r | |
xanthine + NAD+ + H2O | ping-pong reaction mechanism | Gallus gallus | urate + NADH | - |
r | |
xanthine + O2 + H2O | low activity | Gallus gallus | urate + O2- + 2 H+ | - |
r | |
xanthine + phenazine methosulfate + H2O | - |
Gallus gallus | urate + ? | - |
r | |
xanthine + pyridinealdehyde-NAD+ + H2O | 53% of the activity compared to NAD+, low reverse activity | Gallus gallus | urate + pyridinealdehyde-NADH | - |
r | |
xanthine + thio-NAD+ + H2O | same activity compared to NAD+ | Gallus gallus | urate + thio-NADH | - |
r | |
xanthine + trinitrobenzenesulfonate + H2O | - |
Gallus gallus | urate + ? | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Gallus gallus | |
additional information | molybdoironflavoprotein: 1 : 1 : 4 ratio of molybdenum to FAD to iron | Gallus gallus | |
NAD+ | - |
Gallus gallus | |
NADH | - |
Gallus gallus |