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Literature summary for 1.17.1.4 extracted from

  • Schräder, T.; Rienhöfer, A.; Andreesen, J.R.
    Selenium-containing xanthine dehydrogenase from Eubacterium barkeri (1999), Eur. J. Biochem., 264, 862-871.
    View publication on PubMed
Search for more literature for 1.17.1.4

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxypyrazolo(3,4-d)pyrimidine 95% inhibition of hypoxanthine-NADP+-activity at 1 mM; i.e. allopurinol Eubacterium barkeri
6-Mercaptopurine 90% inhibition of hypoxanthine-NADP+-activity at 1 mM Eubacterium barkeri
adenine 96% inhibition of hypoxanthine-NADP+-activity at 1 mM Eubacterium barkeri
arsenite 50% inhibition of hypoxanthine-NADP+-activity at 1 mM Eubacterium barkeri
KCN addition of selenide in the presence of dithionite reactivates the inhibited enzyme Eubacterium barkeri
Sodium dithionite reduction of enzyme Eubacterium barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 NADP+ electron donor: hypoxanthine Eubacterium barkeri
0.067
-
 xanthine electron acceptor: NADP+ Eubacterium barkeri
0.21
-
 hypoxanthine electron acceptor: NADP+ Eubacterium barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Iron-sulfur-center 2 Fe2-S2-clusters Eubacterium barkeri
molybdenum-center
-
 Eubacterium barkeri
Se 3.4 g-atom per mol enzyme Eubacterium barkeri
Tungsten 3.4 g-atom per mol of enzyme Eubacterium barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE Eubacterium barkeri
30000
-
 tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE Eubacterium barkeri
81300
-
 tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE Eubacterium barkeri
530000
-
 gel filtration Eubacterium barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NADP+ + H2O Eubacterium barkeri strict specificity for NADP+ xanthine + NADPH
-
 ?
NADPH + O2 Eubacterium barkeri high NADPH oxidase activity NADP+ + O2- + H+
-
 ?
purine + NADP+ + H2O Eubacterium barkeri 60% of the activity compared to hypoxanthine ? + NADPH
-
 ?
xanthine + NADP+ + H2O Eubacterium barkeri strict specificity for NADP+ urate + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Eubacterium barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Eubacterium barkeri

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by xanthine, selenite and molybdate Eubacterium barkeri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
164
-
 hypoxanthine-NADP+-assay Eubacterium barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoimidazole-5-carboxamide + NADP+ + H2O 38% of the activity compared to hypoxanthine-NADP+ Eubacterium barkeri ? + NADPH
-
 ?
hypoxanthine + NADP+ + H2O strict specificity for NADP+ Eubacterium barkeri xanthine + NADPH
-
 ?
N-methylnicontinamide + NADP+ + H2O low activity, only a substrate at pH values above 8.0 Eubacterium barkeri ? + NADPH
-
 ?
NADPH + electron acceptor + H2O electron acceptors: 2,6-dichlorophenolindophenol, methyl viologen, benzyl viologen, methylene blue Eubacterium barkeri NADP+ + reduced electron acceptor
-
 ?
NADPH + O2 high NADPH oxidase activity Eubacterium barkeri NADP+ + O2- + H+
-
 ?
purine + NADP+ + H2O 60% of the activity compared to hypoxanthine Eubacterium barkeri ? + NADPH
-
 ?
xanthine + NADP+ + H2O strict specificity for NADP+ Eubacterium barkeri urate + NADPH
-
 ?

Subunits

Subunits Comment Organism
dodecamer tetramer of alpha/beta/gamma-protomers, alpha4, 4 * 81300 + beta4, 4 * 30000 + gamma4, 4 * 17500, SDS-PAGE Eubacterium barkeri

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Eubacterium barkeri
additional information molybdoironflavoprotein: 17.5 mol iron, 18.4 mol acid-labile sulfur, 2.3 mol molybdenum, 1.1 mol tungsten, 0.95 mol selenium Eubacterium barkeri