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Literature summary for 1.17.1.4 extracted from

  • Kim, J.M.; Schmid, R.D.
    Purification and characterization of a microbial xanthine dehydrogenase highly active towards hypoxanthine (1989), GBF Monogr. , Biosens. Appl. Med. , Environ. Prot. Process Control, 13, 421-424.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
hypoxanthine
-
Pseudomonas putida
0.052
-
NAD+
-
Pseudomonas putida
0.064
-
xanthine
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
x * 92000 + x * 46000, SDS-PAGE Pseudomonas putida
92000
-
x * 92000 + x * 46000, SDS-PAGE Pseudomonas putida
350000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + NAD+ + H+ + O2- + H2O Pseudomonas putida more rapidly oxidized than xanthine xanthine + NADH + H2O2
-
?
purine + NAD+ + H2O Pseudomonas putida poor substrate ? + NADH
-
?
xanthine + NAD+ + H2O Pseudomonas putida
-
urate + NADH
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Pseudomonas putida

Source Tissue

Source Tissue Comment Organism Textmining
cell culture inducible by hypoxanthine Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80.2
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hypoxanthine + NAD+ + H+ + O2- + H2O more rapidly oxidized than xanthine Pseudomonas putida xanthine + NADH + H2O2
-
?
purine + NAD+ + H2O poor substrate Pseudomonas putida ? + NADH
-
?
xanthine + NAD+ + H2O
-
Pseudomonas putida urate + NADH
-
?

Subunits

Subunits Comment Organism
? x * 92000 + x * 46000, SDS-PAGE Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+ cannot be replaced by NADP+ Pseudomonas putida