KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
hypoxanthine | - |
Pseudomonas putida | |
0.052 | - |
NAD+ | - |
Pseudomonas putida | |
0.064 | - |
xanthine | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 92000 + x * 46000, SDS-PAGE | Pseudomonas putida |
92000 | - |
x * 92000 + x * 46000, SDS-PAGE | Pseudomonas putida |
350000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H+ + O2- + H2O | Pseudomonas putida | more rapidly oxidized than xanthine | xanthine + NADH + H2O2 | - |
? | |
purine + NAD+ + H2O | Pseudomonas putida | poor substrate | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | Pseudomonas putida | - |
urate + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography techniques | Pseudomonas putida |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | inducible by hypoxanthine | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80.2 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + NAD+ + H+ + O2- + H2O | more rapidly oxidized than xanthine | Pseudomonas putida | xanthine + NADH + H2O2 | - |
? | |
purine + NAD+ + H2O | poor substrate | Pseudomonas putida | ? + NADH | - |
? | |
xanthine + NAD+ + H2O | - |
Pseudomonas putida | urate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 92000 + x * 46000, SDS-PAGE | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cannot be replaced by NADP+ | Pseudomonas putida |