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Literature summary for 1.16.3.1 extracted from

  • Griffiths, T.A.; Mauk, A.G.; MacGillivray, R.T.
    Recombinant expression and functional characterization of human hephaestin: a multicopper oxidase with ferroxidase activity (2005), Biochemistry, 44, 14725-14731.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
Ferrous ammonium sulfate pH 5.0, 22°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper 3.1 atoms per protein molecule Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
121000
-
x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens
129600
-
x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens
143000
-
x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
multicopper oxidase hephaestin, with additional ferroxidase activity. Expression in baby hamster kidney cells
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content of 7.7%, no high mannose oligosaccharides Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
after expression in baby hamster kidney cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
apotransferrin + Fe2+ + O2
-
Homo sapiens diferric transferrin + H2O
-
?
ferrous ammonium sulfate + O2
-
Homo sapiens ? + H2O
-
?

Subunits

Subunits Comment Organism
? x * 129600, MALDI-TOF of recombinant glycoprotein, x * 143000, SDS-PAGE of recombinant protein, x * 121000, SDS-PAGE of PNGase F-treated protein Homo sapiens

Synonyms

Synonyms Comment Organism
hephaestin
-
Homo sapiens