Literature summary for 1.16.1.9 extracted from

Miethke, M.; Hou, J.; Marahiel, M.
The siderophore-interacting protein YqjH acts as a ferric reductase in different iron assimilation pathways of Escherichia coli (2011), Biochemistry, 50, 10951-10964.

Search for more literature for 1.16.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K55A	the mutant shows strongly reduced activity compared to the wild type enzyme	Escherichia coli
R130A	the mutant shows strongly reduced activity compared to the wild type enzyme	Escherichia coli
R246A	the variant is rather unstable, showing precipitate formation and cofactor release during protein concentration	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0004	-	Fe(III)-enterobactin	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.0014	-	Fe(III)-vibriobactin	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.0018	-	Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.0042	-	Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.0059	-	Fe(III)-enterobactin	mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.0078	-	Fe(III)-enterobactin	mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.0134	-	Fe(III)-dicitrate	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.036	-	Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3	mutant enzyme R130A in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.04	-	Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3	mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.048	-	Fe(III)-aerobactin	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.051	-	Fe(III)-dicitrate	mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.066	-	Fe(III)-dicitrate	mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Strep-Tactin column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3 + NADPH + H+	-	Escherichia coli	Fe(II) + (2,3-dihydroxybenzoyl-Gly-Thr)3 + NADP+	-	?
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 + NADPH + H+	-	Escherichia coli	Fe(II) + (2,3-dihydroxybenzoyl-L-serine)3 + NADP+	-	?
Fe(III)-aerobactin + NADPH + H+	-	Escherichia coli	Fe(II) + aerobactin + NADP+	-	?
Fe(III)-bacillibactin + NADPH + H+	-	Escherichia coli	Fe(II) + bacillibacitin + NADP+	-	?
Fe(III)-dicitrate + NADPH + H+	-	Escherichia coli	Fe(II) + citrate + NADP+	-	?
Fe(III)-enterobactin + NADPH + H+	-	Escherichia coli	Fe(II) + enterobactin + NADP+	-	?
Fe(III)-vibriobactin + NADPH + H+	-	Escherichia coli	Fe(II) + vibriobactin + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
ferric siderophore reductase	-	Escherichia coli
YqjH	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli
NADPH	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	the absence of YqjH slows growth	Escherichia coli
metabolism	YqjH represents a redox factor that enhances the efficiency of ferric iron assimilation during siderophore-dependent iron homeostasis and enhances siderophore utilization in different iron acquisition pathways, including assimilation of low-potential ferric substrates that are not reduced by common cellular cofactors	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.02	-	Fe(III)-aerobactin	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.032	-	Fe(III)-enterobactin	mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.076	-	Fe(III)-enterobactin	mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.091	-	Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3	mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.132	-	Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3	mutant enzyme R130A in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.139	-	Fe(III)-dicitrate	mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
0.271	-	Fe(III)-dicitrate	mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
1.268	-	Fe(III)-vibriobactin	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
5.427	-	Fe(III)-dicitrate	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
8.211	-	Fe(III)-enterobactin	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
9.193	-	Fe(III)-(N-2,3-dihydroxybenzoyl-Gly-Thr)3	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli
23.35	-	Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3	wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C	Escherichia coli

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Release 2023.1 (January 2023)