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Literature summary for 1.16.1.9 extracted from

  • Bamford, V.; Armour, M.; Mitchell, S.; Cartron, M.; Andrews, S.; Watson, K.
    Preliminary X-ray diffraction analysis of YqjH from Escherichia coli: A putative cytoplasmic ferri-siderophore reductase (2008), Acta Crystallogr. Sect. F, 64, 792-796.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.16.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M succinic acid-sodium phosphate dibasic-glycine buffer pH 4.0 and 18% (w/v) PEG 1500 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Escherichia coli 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32400
-
 x * 32400, His-tagged enzyme, SDS-PAGE Escherichia coli
32412
-
 x * 32412, His-tagged enzyme, calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-Sepharose column chromatography and DEAE Sepharose column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an Fe(III)-siderophore + NADPH
-
 Escherichia coli Fe(II) + an apo-siderophore + NADP+ + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 32400, His-tagged enzyme, SDS-PAGE Escherichia coli
? x * 32412, His-tagged enzyme, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
ferri-siderophore reductase
-
 Escherichia coli
YqjH
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
FAD contains FAD Escherichia coli
NADPH
-
 Escherichia coli