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Literature summary for 1.16.1.7 extracted from

  • Jeong, J.; Cohu, C.; Kerkeb, L.; Pilon, M.; Connolly, E.L.; Guerinot, M.L.
    Chloroplast Fe(III) chelate reductase activity is essential for seedling viability under iron limiting conditions (2008), Proc. Natl. Acad. Sci. USA, 105, 10619-10624.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
agriculture ferric reductase oxidase 7 is a chloroplast Fe(III) chelate reductase required for survival under ironlimiting conditions, for efficient photosynthesis, and for proper chloroplast iron acquisition in young seedlings Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression of ferric reductase oxidase FRO6-GFP or FRO7-GFP in yeast Saccharomyces cerevisiae
transient expression of yeast ferric reductase oxidase FRO6-GFP or FRO7-GFP in Arabidopsis protoplasts, generation of transgenic plants expressing glucuronidase reporter gene fused to the FRO7 promoter, generation of Fro7 mutants missing the Fe(III) chelate reductase Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast membrane Arabidopsis thaliana 9507
-
mitochondrion FRO7 Saccharomyces cerevisiae 5739
-
plasma membrane FRO6 Saccharomyces cerevisiae 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information ferric reductase oxidase 7 is a chloroplast Fe(III) chelate reductase required for survival under ironlimiting conditions, for efficient photosynthesis, and for proper chloroplast iron acquisition in young seedlings Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
wild type and transgenic plants expressing a yeast ferric chelate reductase gene, mutants missing the Fe(III) chelate reductase
-
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
plant
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Fe(II) + NAD+ chloroplats with 300 micromolar ferrozine and 100 micromolar Fe-EDTA in Hepes-sorbitol buffer, pH 7.3 Arabidopsis thaliana 2 Fe(III) + NADH + H+
-
?
2 Fe2+ + NAD+ transgenic FRO6 yeast has higher Fe3+ chelate reductase activity, Fe3+ chelate reductase activity of FRO7-expressing cells does not differ from the basal level observed in the vector-only control cells Saccharomyces cerevisiae 2 Fe3+ + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
Fe(III) chelate reductase
-
Saccharomyces cerevisiae
Fe(III) chelate reductase
-
Arabidopsis thaliana
ferric reductase oxidase
-
Saccharomyces cerevisiae
ferric reductase oxidase
-
Arabidopsis thaliana
FRO6
-
Saccharomyces cerevisiae
FRO7
-
Saccharomyces cerevisiae
FRO7
-
Arabidopsis thaliana