Protein Variants | Comment | Organism |
---|---|---|
additional information | cloning and expression of catalytic core and N-terminal domain of enzyme as separate proteins. the N-terminal domain NmerA is a stable, soluble protein that binds 1 Hg2+ per domain and delivers it to the catalytic core at kinetically competent rates | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
Hg2+ | recombinant catalytic core, presence of N-terminal sequence NmerA, 25°C, pH 7.3 | Bacillus sp. (in: Bacteria) | |
0.297 | - |
Hg2+ | full-length enzyme, 25°C, pH 7.3 | Bacillus sp. (in: Bacteria) | |
1.177 | - |
Hg2+ | recombinant catalytic core, 25°C, pH 7.3 | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) RC607 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hg2+ + NADPH | - |
Bacillus sp. (in: Bacteria) | Hg + NADP+ + H+ | - |
? | |
Hg2+ + NADPH | - |
Bacillus sp. (in: Bacteria) RC607 | Hg + NADP+ + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | N-terminal domain NmerA participates in acquisition and delivery of Hg2+ to the catalytic core during thr reduction catalyzed by full-length enzyme | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
Hg2+ | recombinant catalytic core, 25°C, pH 7.3 | Bacillus sp. (in: Bacteria) | |
9 | - |
Hg2+ | full-length enzyme, 25°C, pH 7.3 | Bacillus sp. (in: Bacteria) | |
12 | - |
Hg2+ | recombinant catalytic core, presence of N-terminal sequence NmerA, 25°C, pH 7.3 | Bacillus sp. (in: Bacteria) |