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Literature summary for 1.15.1.2 extracted from

  • Almeida, R.M.; Turano, P.; Moura, I.; Moura, J.J.; Pauleta, S.R.
    Superoxide reductase: different interaction modes with its two redox partners (2013), ChemBioChem, 14, 1858-1866.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21(DE3) Megalodesulfovibrio gigas

Inhibitors

Inhibitors Comment Organism Structure
Zn-substituted rubredoxin generation of Zn-substituted rubredoxin, which is diamagnetic and redox inactive, titration of Zn-rubredoxin with superoxide reductase, overview Megalodesulfovibrio gigas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, overview Megalodesulfovibrio gigas

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Megalodesulfovibrio gigas 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the class II enzyme has a single redox catalytic center consisting of an Fe atom bound to four nitrogen atoms from histidine side chains in the equatorial plane and to one cysteine sulfur in the axial plane Megalodesulfovibrio gigas
additional information ionic strength dependence of superoxide-mediated rubredoxin oxidation Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
-
Megalodesulfovibrio gigas

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced desulforedoxin + superoxide + 2 H+ Megalodesulfovibrio gigas
-
desulforedoxin + H2O2
-
?
reduced rubredoxin + superoxide + 2 H+ Megalodesulfovibrio gigas
-
rubredoxin + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Megalodesulfovibrio gigas
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, anion exchange chromatography, and gel filtration Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced desulforedoxin + superoxide + 2 H+
-
Megalodesulfovibrio gigas desulforedoxin + H2O2
-
?
reduced rubredoxin + superoxide + 2 H+
-
Megalodesulfovibrio gigas rubredoxin + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer
-
Megalodesulfovibrio gigas

Synonyms

Synonyms Comment Organism
SOR
-
Megalodesulfovibrio gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
ionic strength dependence of superoxide-mediated rubredoxin oxidation Megalodesulfovibrio gigas
7.6
-
assay at Megalodesulfovibrio gigas

Cofactor

Cofactor Comment Organism Structure
desulforedoxin endogenous, contains Zn2+, the protein is able to transfer electrons to superoxide reductase with a maximum kapp of 31/min at an ionic strength of 57 mM, the enzyme complex with superoxide reductase is not detected by chemical shift perturbation Megalodesulfovibrio gigas
additional information titration of Zn-rubredoxin with superoxide reductase, overview. NMR competition assay between desulforedoxin and rubredoxin for binding to SOR, overview Megalodesulfovibrio gigas
rubredoxin endogenous. A monomeric, non-heme iron protein that contains a tetrahedral FeS4 metal center. The rubredoxin surface involved in the electron transfer complex with superoxide reductase comprises the solvent-exposed hydrophobic residues in the vicinity of its metal center, Cys9, Gly10, Cys42, Gly43, and Ala44. Kd of 0.003 mM at 59 mM ionic strength by NMR. Model structure of superoxide reductase-rubredoxin complex, overview Megalodesulfovibrio gigas

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0004
-
pH 7.6, temperature not specified in the publication Megalodesulfovibrio gigas Zn-substituted rubredoxin

General Information

General Information Comment Organism
evolution the enzyme belongs to the class II superoxide reductase family Megalodesulfovibrio gigas
additional information model structure of SOR-rubredoxin complex, docking simulations, overview Megalodesulfovibrio gigas