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Literature summary for 1.15.1.2 extracted from

  • Jenney, F.E., Jr.; Verhagen, M.F.J.M.; Cui, X.; Adams, M.W.W.
    Anaerobic microbes: oxygen detoxification without superoxide dismutase (1999), Science, 286, 306-309.
    View publication on PubMed
Search for more literature for 1.15.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+/Fe3+ 0.5 iron atoms/mol subunit Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
 x * 14000, SDS-PAGE Pyrococcus furiosus
14323
-
 x * 14323, deduced from nucleotide sequence Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
reduced rubredoxin + superoxide + H+ Pyrococcus furiosus rubredoxin is assumed to be the physiological electron carrier oxidized rubredoxin + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
 hyperthermophilic anaerobe
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
reduced cytochrome c + superoxide + H+
-
 Pyrococcus furiosus cytochrome c + H2O2
-
 ?
reduced rubredoxin + superoxide + H+ rubredoxin is assumed to be the physiological electron carrier Pyrococcus furiosus rubredoxin + H2O2
-
 ?
reduced rubredoxin + superoxide + H+ rubredoxin is assumed to be the physiological electron carrier Pyrococcus furiosus oxidized rubredoxin + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 14000, SDS-PAGE Pyrococcus furiosus
? x * 14323, deduced from nucleotide sequence Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25
-
 enzyme functions efficiently in vitro at 25°C, which is 75°C below the organism's optimal growth temperature Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
cytochrome c artificial electron carrier Pyrococcus furiosus
rubredoxin assumed to be the phsiological electron carrier Pyrococcus furiosus