Cloned (Comment) | Organism |
---|---|
gene RmFeSOD, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3). Under copper stress experiment, the RmFeSOD-expressing Escherichia coli cells exhibit a better growth than nontransformed bacteria | Rhodotorula mucilaginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Rhodotorula mucilaginosa | |
DMSO | - |
Rhodotorula mucilaginosa | |
Mg2+ | 30% inhibition at 1 mM | Rhodotorula mucilaginosa | |
additional information | no inhibition by DTT. Ba2+, Ca2+, Ni2+, and Fe2+ have no obvious impact to enzyme activity at 1 mM | Rhodotorula mucilaginosa | |
PMSF | - |
Rhodotorula mucilaginosa | |
SDS | 70% inhibition | Rhodotorula mucilaginosa | |
Triton X-100 | 70% inhibition | Rhodotorula mucilaginosa | |
Tween-80 | 70% inhibition | Rhodotorula mucilaginosa | |
Urea | - |
Rhodotorula mucilaginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates the enzyme at 1 mM | Rhodotorula mucilaginosa | |
Fe2+ | required, a Fe-SOD | Rhodotorula mucilaginosa | |
Fe3+ | activates the enzyme at 1 mM | Rhodotorula mucilaginosa | |
Mn2+ | activates the enzyme at 1 mM | Rhodotorula mucilaginosa | |
Zn2+ | activates the enzyme at 1 mM | Rhodotorula mucilaginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49300 | - |
recombinant His-tagged enzyme, gel filtration | Rhodotorula mucilaginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | Rhodotorula mucilaginosa | - |
O2 + H2O2 | - |
? | |
2 superoxide + 2 H+ | Rhodotorula mucilaginosa AN5 | - |
O2 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | A0A1L5JYH9 | - |
- |
Rhodotorula mucilaginosa AN5 | A0A1L5JYH9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Rhodotorula mucilaginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 superoxide + 2 H+ | - |
Rhodotorula mucilaginosa | O2 + H2O2 | - |
? | |
2 superoxide + 2 H+ | - |
Rhodotorula mucilaginosa AN5 | O2 + H2O2 | - |
? | |
additional information | enzyme activity measurement by reduction of NBT | Rhodotorula mucilaginosa | ? | - |
? | |
additional information | enzyme activity measurement by reduction of NBT | Rhodotorula mucilaginosa AN5 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 23500, about, sequence calculation, 2 * 31000, recombinant His-tagged enzyme, SDS-PAGE | Rhodotorula mucilaginosa |
Synonyms | Comment | Organism |
---|---|---|
iron SOD type | - |
Rhodotorula mucilaginosa |
RmFeSOD | - |
Rhodotorula mucilaginosa |
SOD | - |
Rhodotorula mucilaginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rhodotorula mucilaginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | recombinant enzyme, 1 h, about 90% activity remaining, completely stable for 45 min | Rhodotorula mucilaginosa |
50 | - |
recombinant enzyme, 1 h, 80% activity remaining | Rhodotorula mucilaginosa |
60 | - |
recombinant enzyme, 1 h, 60% activity remaining | Rhodotorula mucilaginosa |
70 | - |
recombinant enzyme, 1 h, 20% activity remaining | Rhodotorula mucilaginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | - |
Rhodotorula mucilaginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 11 | activity range, 75% of maximal activity at pH 1.0, 45% at pH 11.0, profile overview | Rhodotorula mucilaginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 9 | recombinant RmFeSOD shows good pH stability in the pH 1.0-9.0 after 1 h incubation | Rhodotorula mucilaginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodotorula mucilaginosa | sequence calculation | - |
7.89 |
General Information | Comment | Organism |
---|---|---|
additional information | the conserved histidine residues (His41, His89, His179), aspartate residue (Asp175), and WEHAYY domain play an important role in the active site of the Fe-binding sites | Rhodotorula mucilaginosa |
physiological function | enzyme RmFeSOD plays an important role in the adaptability of heavy metals | Rhodotorula mucilaginosa |