Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a fusion protein between the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 and superoxide dismutase from Sulfolobus solfataricus. The recombinant protein exhibits improved thermophilicity, higher working temperature, improved thermostability, broader pH stability, and enhanced tolerance to inhibitors and organic media without any alterations in its oligomerization state. The N-terminal domain is a good candidate for improving stability of both mesophilic and thermophilic superoxide dismutase from either bacteria or archaea via simple genetic manipulation | Geobacillus thermodenitrificans |
additional information | construction of a fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2. The recombinant protein exhibits improved thermophilicity, higher working temperature, improved thermostability, broader pH stability, and enhanced tolerance to inhibitors and organic media without any alterations in its oligomerization state | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | A4ISC5 | - |
- |
Geobacillus thermodenitrificans NG80-2 | A4ISC5 | - |
- |
Saccharolobus solfataricus | P80857 | - |
- |
Saccharolobus solfataricus DSM 1617 | P80857 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
GTNG_2884 | - |
Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type | Saccharolobus solfataricus |
60 | - |
fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
5 h, 40% loss of activity for wild-type, 13% loss of activity for fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 | Saccharolobus solfataricus |
105 | - |
half-life 2.1 h for wild-type, 5.7 h for fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
both wild-type and fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2 | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | fusion protein with the N-terminal domain of superoxide dismutase from Geobacillus thermodenitrificans NG80-2, more than 90% of initial activity | Saccharolobus solfataricus |
5 | - |
wild-type, unstable above | Saccharolobus solfataricus |