Cloned (Comment) | Organism |
---|---|
gene sod, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Geobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Geobacillus sp. | |
Ba2+ | inhibitory at 1 mM | Geobacillus sp. | |
Ca2+ | inhibitory at 1 mM | Geobacillus sp. | |
CHAPS | - |
Geobacillus sp. | |
Co2+ | inhibitory at 1 mM | Geobacillus sp. | |
Cu2+ | 50% inhibition at 1 mM | Geobacillus sp. | |
dithiothreitol | - |
Geobacillus sp. | |
EDTA | strong inhibition at 10 mM | Geobacillus sp. | |
Fe2+ | inhibitory at 1 mM | Geobacillus sp. | |
guanidine hydrochloride | - |
Geobacillus sp. | |
Mg2+ | inhibitory at 1 mM | Geobacillus sp. | |
additional information | insensitivity of the recombinant enzyme to both KCN and H2O2 | Geobacillus sp. | |
Ni2+ | inhibitory at 1 mM | Geobacillus sp. | |
phenylmethylsulfonyl fluoride | - |
Geobacillus sp. | |
SDS | - |
Geobacillus sp. | |
Triton X-100 | - |
Geobacillus sp. | |
Tween 20 | - |
Geobacillus sp. | |
Urea | - |
Geobacillus sp. | |
Zn2+ | inhibitory at 1 mM | Geobacillus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | a Mn-SOD, Mn2+ activates, the manganese-binding site is formed by conserved residues His260, His308, Asp392, and His396 | Geobacillus sp. | |
additional information | no enzyme activity with Fe2+-reconstituted enzyme | Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50230 | - |
2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation | Geobacillus sp. |
59000 | - |
2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation | Geobacillus sp. |
130700 | - |
recombinant His-tagged enzyme, gel filtration | Geobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | T1T1K2 | gene sod | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Geobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1204 | - |
purified recombinant His-tagged enzyme, pH 7.8, 25°C | Geobacillus sp. |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation | Geobacillus sp. |
More | modelling of the three-dimensional structure of SOD monomer, overview | Geobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Geobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Geobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant enzyme, stable | Geobacillus sp. |
80 | - |
purified recombinant enzyme, 1 h, 64% activity remaining | Geobacillus sp. |
90 | - |
purified recombinant enzyme, 1 h, 57% activity remaining | Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Geobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | purified recombinant enzyme, stable | Geobacillus sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus sp. | sequence calculation | - |
4.65 |
General Information | Comment | Organism |
---|---|---|
additional information | Geobacillus sp. EPT3 SOD contains the LPPLPYRYDALEP sequence, which is identical to the conserved amino acid sequence (LPXLPYXXXXLEP) found at the N-terminal region of many Mn-SODs | Geobacillus sp. |