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Literature summary for 1.15.1.1 extracted from

  • Cho, M.H.; Shin, Y.W.; Chun, J.H.; Hong, K.J.; Na, B.K.; Rhie, G.E.; Seong, B.L.; Yoo, C.K.
    Expression and characterization of an iron-containing superoxide dismutase from Burkholderia pseudomallei (2012), J. Microbiol., 50, 1029-1033.
    View publication on PubMed
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene sodB, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Escherichia coli strain BL21(DE3) Burkholderia pseudomallei

Inhibitors

Inhibitors Comment Organism Structure
hydrogen peroxide
-
 Burkholderia pseudomallei
additional information no inhibition by sodium azide or potassium cyanide Burkholderia pseudomallei
paraquat
-
 Burkholderia pseudomallei

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a Fe-SOD Burkholderia pseudomallei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, recombinant His-tagged enzyme, SDS-PAGE Burkholderia pseudomallei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 O2.- + 2 H+ Burkholderia pseudomallei
-
 O2 + H2O2
-
 ?
2 O2.- + 2 H+ Burkholderia pseudomallei 1106a
-
 O2 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Burkholderia pseudomallei A3NS93 isolated from a melioidosis patient in Korea
-
Burkholderia pseudomallei 1106a A3NS93 isolated from a melioidosis patient in Korea
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Burkholderia pseudomallei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 O2.- + 2 H+
-
 Burkholderia pseudomallei O2 + H2O2
-
 ?
2 O2.- + 2 H+
-
 Burkholderia pseudomallei 1106a O2 + H2O2
-
 ?

Subunits

Subunits Comment Organism
? x * 25000, recombinant His-tagged enzyme, SDS-PAGE Burkholderia pseudomallei

Synonyms

Synonyms Comment Organism
Fe-SOD
-
 Burkholderia pseudomallei
iron-containing superoxide dismutase
-
 Burkholderia pseudomallei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Burkholderia pseudomallei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 purified recombinant His-tagged enzyme, 48 h, over 80% activity remaining Burkholderia pseudomallei
50
-
 purified recombinant His-tagged enzyme, completely stable for 6 h, after 48 h 30% activity remaining Burkholderia pseudomallei
70
-
 purified recombinant His-tagged enzyme, 20% activity remaining after 1 h, inactivation after 3 h Burkholderia pseudomallei
100
-
 purified recombinant His-tagged enzyme, 5% activity remaining after 1 h, inactivation after 2 h Burkholderia pseudomallei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Burkholderia pseudomallei

pH Range

pH Minimum pH Maximum Comment Organism
7 12 over 70% of maximal activity within this range Burkholderia pseudomallei