Cloned (Comment) | Organism |
---|---|
expression of His-tagged SOD in Escherichia coli strain BL21(DE3) | Streptococcus mutans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inactivation kinetics of rSmSOD by hydrogen peroxide, overview | Streptococcus mutans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the recombinant SOD binds either Fe or Mn as a metal co-factor, with a consistent preference for Fe accommodation. But differently from the significant preference for Fe displays by the enzyme in the binding reaction, its Mn-form is 71fold more active compared to the Fe-form | Streptococcus mutans | |
Mn | the recombinant SOD binds either Fe or Mn as a metal co-factor, with a consistent preference for Fe accommodation. But differently from the significant preference for Fe displays by the enzyme in the binding reaction, its Mn-form is 71fold more active compared to the Fe-form | Streptococcus mutans | |
additional information | different values of the Mn/Fe ratio in the active site prove that the type of metal is crucial for the regulation of the activity of recombinant SmSOD | Streptococcus mutans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus mutans | - |
- |
- |
Streptococcus mutans UA159 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged SOD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptococcus mutans |
Synonyms | Comment | Organism |
---|---|---|
SOD | - |
Streptococcus mutans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | assay at | Streptococcus mutans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
71 | 73 | half-inactivation occurring after 10 min exposure at 71-73°C, depending on the bound metal | Streptococcus mutans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Streptococcus mutans |
General Information | Comment | Organism |
---|---|---|
additional information | different values of the Mn/Fe ratio in the active site prove that the type of metal is crucial for the regulation of the activity of recombinant SmSOD, cambialistic nature of SmSOD, overview | Streptococcus mutans |