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Literature summary for 1.15.1.1 extracted from

  • De Vendittis, A.; Amato, M.; Mickniewicz, A.; Parlato, G.; De Angelis, A.; Castellano, I.; Rullo, R.; Riccitiello, F.; Rengo, S.; Masullo, M.; De Vendittis, E.
    Regulation of the properties of superoxide dismutase from the dental pathogenic microorganism Streptococcus mutans by iron- and manganese-bound co-factor (2010), Mol. Biosyst., 6, 1973-1982.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged SOD in Escherichia coli strain BL21(DE3) Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
H2O2 inactivation kinetics of rSmSOD by hydrogen peroxide, overview Streptococcus mutans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe the recombinant SOD binds either Fe or Mn as a metal co-factor, with a consistent preference for Fe accommodation. But differently from the significant preference for Fe displays by the enzyme in the binding reaction, its Mn-form is 71fold more active compared to the Fe-form Streptococcus mutans
Mn the recombinant SOD binds either Fe or Mn as a metal co-factor, with a consistent preference for Fe accommodation. But differently from the significant preference for Fe displays by the enzyme in the binding reaction, its Mn-form is 71fold more active compared to the Fe-form Streptococcus mutans
additional information different values of the Mn/Fe ratio in the active site prove that the type of metal is crucial for the regulation of the activity of recombinant SmSOD Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
-
-
Streptococcus mutans UA159
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged SOD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptococcus mutans

Synonyms

Synonyms Comment Organism
SOD
-
Streptococcus mutans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30 assay at Streptococcus mutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
71 73 half-inactivation occurring after 10 min exposure at 71-73°C, depending on the bound metal Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Streptococcus mutans

General Information

General Information Comment Organism
additional information different values of the Mn/Fe ratio in the active site prove that the type of metal is crucial for the regulation of the activity of recombinant SmSOD, cambialistic nature of SmSOD, overview Streptococcus mutans