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Literature summary for 1.15.1.1 extracted from

  • Zhang, L.Q.; Guo, F.X.; Xian, H.Q.; Wang, X.J.; Li, A.N.; Li, D.C.
    Expression of a novel thermostable Cu, Zn-superoxide dismutase from Chaetomium thermophilum in Pichia pastoris and its antioxidant properties (2011), Biotechnol. Lett., 33, 1127-1132.
    View publication on PubMed

Application

Application Comment Organism
biotechnology transfection of the Ctsod gene may have great potential for the genetic improvement of salt tolerance in plants Thermochaetoides thermophila

Cloned(Commentary)

Cloned (Comment) Organism
gene Ctsod, DNA and amino acid sequence determination and analysis, phylogenetic tree, subcloning in Escherichia coli strains DH5a and JM109, expression in Pichia pastoris strain GS115, the recombinant yeast exhibit higher stress resistance than the control yeast cells to salt and superoxide-generating agents, such as paraquat and menadione Thermochaetoides thermophila

Inhibitors

Inhibitors Comment Organism Structure
H2O2
-
Thermochaetoides thermophila
KCN
-
Thermochaetoides thermophila
additional information no inhibition by NaN3 Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a CuZn-superoxide dismutase Thermochaetoides thermophila
Zn2+ a CuZn-superoxide dismutase Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila Q1HEQ0 gene Ctsod
-
Thermochaetoides thermophila HSAUP072651 Q1HEQ0 gene Ctsod
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Cu, ZnSOD from Pichia pastoris strain GS115 by anion exchange chromatography Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2170
-
purified recombinant enzyme, pH 6.5, 60°C Thermochaetoides thermophila

Synonyms

Synonyms Comment Organism
CtSOD
-
Thermochaetoides thermophila
Cu, Zn-superoxide dismutase
-
Thermochaetoides thermophila
Cu, ZnSOD
-
Thermochaetoides thermophila
SOD
-
Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
recombinant enzyme Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
purified recombinant enzyme, retains 65% of the maximum activity at 70°C for 60 min Thermochaetoides thermophila
80
-
purified recombinant enzyme, half-life is 22 min Thermochaetoides thermophila
90
-
purified recombinant enzyme, half-life is 7 min Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
recombinant enzyme Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
5 10 activity profile, overview Thermochaetoides thermophila

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the purified recombinant enzyme is relatively stable at pH 5.0-9.0, and retains more than 70% of full activity at pH 5.5-8.0 Thermochaetoides thermophila