Protein Variants | Comment | Organism |
---|---|---|
additional information | polysialylation of SOD, method development and optimization, overview. Optimal conditions for the cross-linking reaction are the ratio of polysialic acid and SOD of 40:1 with a reaction time of 24 h. Under this condition, the average cross-linking ratio is 3.9 and average molecular weight was 95 kDa derived from the molecular weight of polysialic acid with 16.2 kDa and CuZn-SOD with 32 kDa. The molecular size of the polysialylated enzyme was about 90-100 kDa, enhancement of hydratability of SOD through polysialylation, analysis by atomic force microscopy, overview | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the native enzyme is degraded by pepsin and trypsin, while the polysialylated SOD is resistant to pepsin and trypsin | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a CuZn-superoxide dismutase | Sus scrofa | |
Zn2+ | a CuZn-superoxide dismutase | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, native enzyme, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | SOD enzyme activity measurement is based on the inhibition of nitroblue tetrazolium reduction by superoxide radical generated by xanthine/xanthine oxidase | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, native enzyme, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
CuZn-superoxide dismutase | - |
Sus scrofa |
SOD | - |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | the initial activities of the polysialyated enzyme show 35-55% higher than those of the native enzyme after incubation at 70°C, and 31-45% at 80°C, the native enzyme is almost inactivated after incubation for 3 h, while the polysialylated SOD still has 49-61% residual activities | Sus scrofa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 9 | the pH stability of the enzyme is enhanced by the polysialylation, after 1 h at pH 2-3, the residual enzyme activity of polysialylated SOD is 64-74%, while the native SOD shows quickly decreased activity to 20-38% | Sus scrofa |