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Literature summary for 1.15.1.1 extracted from
- Identification and characterization of a mitochondrial iron-superoxide dismutase of Cryptosporidium parvum (2008), Parasitol. Res., 103, 787-795.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparison, His-tagged enzyme expression in Escherichia coli strains DH5alpha and M15 | Cryptosporidium parvum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Cryptosporidium parvum |  |
| additional information | no inhibition by NaN3 and KCN | Cryptosporidium parvum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | the enzyme contains an N-terminal mitochondrial targeting sequence | Cryptosporidium parvum | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | - |
Cryptosporidium parvum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
2 * 26000, recombinant enzyme, SDS-PAGE | Cryptosporidium parvum |
| 50000 | - |
recombinant enzyme, gel filtration | Cryptosporidium parvum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cryptosporidium parvum | Q5UVY7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strains DH5alpha and M15 by nickel affinity chromatography | Cryptosporidium parvum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 26000, recombinant enzyme, SDS-PAGE | Cryptosporidium parvum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fe-SOD | - |
Cryptosporidium parvum |
| iron-superoxide dismutase | - |
Cryptosporidium parvum |
| mtSOD | - |
Cryptosporidium parvum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 70 | the recombinant purified enzyme is fairly stable at 4°C and 37°C, but is rapidly inactivated at 50°C and 70°C | Cryptosporidium parvum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Cryptosporidium parvum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | optimum pH-range | Cryptosporidium parvum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the recombinant purified enzyme is highly stable at neutral and alkaline pHs but is relatively unstable in acidic condition | Cryptosporidium parvum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | SOD detoxifies the highly reactive superoxide anions to hydrogen peroxide and molecular oxygen | Cryptosporidium parvum |
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