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Literature summary for 1.15.1.1 extracted from

  • Yamano, S.; Maruyama, T.
    An azide-insensitive superoxide dismutase from a hyperthermophilic archaeon, Sulfolobus solfataricus (1999), J. Biochem., 125, 186-193.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and anaylsis, expression of wild-type and mutant in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
Y88F site-directed mutagenesis, substitution of Tyr88 to Phe does not affect the metal specificity of the enzyme Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
fluoride recombinant Fe-reconstituted SOD Saccharolobus solfataricus
H2O2 distinguishes Fe-SOD from Mn-SOD, since it inactivates only Fe-SOD Saccharolobus solfataricus
additional information recombinant Fe-reconstituted SOD is not inhibited by azide, steric hindrance in the substrate funnel of the enzyme prevents the access of N3- but allows O2- and F- access to the active site Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ Fe/Mn-type SOD Saccharolobus solfataricus
Mn2+ Fe/Mn-type SOD Saccharolobus solfataricus
additional information metal content analysis of the recombinant enzyme Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus P80857 DSM 1616
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant from Escherichia coli Saccharolobus solfataricus

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of Fe-SOD from purified recombinant apo-enzyme, apo-enzyme preparation: purified recombinant enzyme is denatured in buffer containing 50 mM acetate buffer, pH 3.8, 6 M guanidine hydrochloride, and 10 mM EDTA for 16 h at 50°C, followed by dialysis against the same buffer containing MnSO4 instead of EDTA, and removal of guanidine hydrochloride in a second dialysis step, followed by gel filtration, overview Saccharolobus solfataricus

Source Tissue

Source Tissue Comment Organism Textmining
additional information Sulfolobus solfataricus grows between 50°C and 87°C with an optimum growing temperature of 87°C, but it neither grows nor survives at 90°C Saccharolobus solfataricus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
reconstituted Mn-SOD Saccharolobus solfataricus
4.3
-
reconstituted Mn-SOD mutant Y88F Saccharolobus solfataricus
481
-
reconstituted Fe-SOD Saccharolobus solfataricus
755
-
reconstituted Fe-SOD mutant Y88F Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
SOD
-
Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
2 h, purified recombinant enzyme, 96% remaining activity, half-life is 33 h, inactivation according to first order kinetics Saccharolobus solfataricus
100
-
purified recombinant enzyme, half-life is 8.7 h, inactivation according to first order kinetics Saccharolobus solfataricus
105
-
purified recombinant enzyme, half-life is 1.5 h, inactivation according to first order kinetics Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Saccharolobus solfataricus