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Literature summary for 1.15.1.1 extracted from

  • Lin, M.W.; Lin, M.T.; Lin, C.T.
    Copper/zinc-superoxide dismutase from lemon cDNA and enzyme stability (2002), J. Agric. Food Chem., 50, 7264-7270.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Cu,Zn-SOD, expression in Escherichia coli as His-tagged protein, DNA and amino acid sequence analysis Citrus limon

General Stability

General Stability Organism
dimeric form is more stable against proteolysis than monomeric form Citrus limon

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+
-
Citrus limon
Zn2+
-
Citrus limon

Organism

Organism UniProt Comment Textmining
Citrus limon Q71S31 Cu,Zn-SOD
-
Citrus limon CuZn-SOD Q71S31 Cu,Zn-SOD
-

Purification (Commentary)

Purification (Comment) Organism
2 forms, 1 dimer and 1 monomer Citrus limon
recombinant His-tagged Cu,Zn-SOD from Escherichia coli Citrus limon

Reaction

Reaction Comment Organism Reaction ID
2 superoxide + 2 H+ = O2 + H2O2 amino acid sequence alignment and comparison Citrus limon
2 superoxide + 2 H+ = O2 + H2O2 A metalloprotein. Enzymes from most eukaryotes contain both copper and zinc, those from mitochondria and most prokaryotes contain manganese or iron. ligand binding site and structure Citrus limon

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Citrus limon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
dimeric form shows 65% higher activity than monomeric form Citrus limon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O2- + H+
-
Citrus limon O2 + H2O2
-
?
O2- + H+
-
Citrus limon CuZn-SOD O2 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer
-
Citrus limon
monomer
-
Citrus limon
More Cu,Zn-SOD exists as 70% dimeric form and 30% monomeric form Citrus limon

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
dimer: half-life 99 min, dimeric form is more stable than monomeric form Citrus limon

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.2 11 Cu,Zn-SOD, stable Citrus limon