Cloned (Comment) | Organism |
---|---|
Cu,Zn-SOD, expression in Escherichia coli as His-tagged protein, DNA and amino acid sequence analysis | Citrus limon |
General Stability | Organism |
---|---|
dimeric form is more stable against proteolysis than monomeric form | Citrus limon |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Citrus limon | |
Zn2+ | - |
Citrus limon |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus limon | Q71S31 | Cu,Zn-SOD | - |
Citrus limon CuZn-SOD | Q71S31 | Cu,Zn-SOD | - |
Purification (Comment) | Organism |
---|---|
2 forms, 1 dimer and 1 monomer | Citrus limon |
recombinant His-tagged Cu,Zn-SOD from Escherichia coli | Citrus limon |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 superoxide + 2 H+ = O2 + H2O2 | amino acid sequence alignment and comparison | Citrus limon | |
2 superoxide + 2 H+ = O2 + H2O2 | A metalloprotein. Enzymes from most eukaryotes contain both copper and zinc, those from mitochondria and most prokaryotes contain manganese or iron. ligand binding site and structure | Citrus limon |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Citrus limon | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
dimeric form shows 65% higher activity than monomeric form | Citrus limon |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Citrus limon | O2 + H2O2 | - |
? | |
O2- + H+ | - |
Citrus limon CuZn-SOD | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Citrus limon |
monomer | - |
Citrus limon |
More | Cu,Zn-SOD exists as 70% dimeric form and 30% monomeric form | Citrus limon |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
dimer: half-life 99 min, dimeric form is more stable than monomeric form | Citrus limon |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.2 | 11 | Cu,Zn-SOD, stable | Citrus limon |