Literature summary for 1.15.1.1 extracted from

Fujii, J.; Myint, T.; Seo, H.G.; Kyanoki, Y.; Ikeda, Y.; Taniguchi, N.
Characterization of wild-type and amyotrophic lateral sclerosis-related mutant Cu,Zn-superoxide dismutases overproduced in baculovirus-infected insect cells (1995), J. Neurochem., 64, 1456-1461.

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Application

Application	Comment	Organism
medicine	investigations with help of mutants to elucidate structure-function relation in familial amyotrophic lateral sclerosis	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
Cu,Zn-SOD, overexpression of wild-type and mutants in Spodoptera frugiperda cells Sf21 via baculovirus infection	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
G41N	Cu,Zn-SOD, site-directed mutagenesis, analogous to mutant found in familial amyotrophic lateral sclerosis, 47% activity compared to the wild-type	Homo sapiens
G85R	Cu,Zn-SOD, site-directed mutagenesis, analogous to mutant found in familial amyotrophic lateral sclerosis, 99% activity compared to the wild-type	Homo sapiens
H43R	Cu,Zn-SOD, site-directed mutagenesis, analogous to mutant found in familial amyotrophic lateral sclerosis, 66% activity compared to the wild-type	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	-	Homo sapiens
Zn2+	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	Cu,Zn-SOD	-
Homo sapiens CuZn-SOD	-	Cu,Zn-SOD	-

Purification (Commentary)

Purification (Comment)	Organism
Cu,Zn-SOD, wild-type and mutants recombinant from Spodoptera frugiperda cells	Homo sapiens

Renatured (Commentary)

Renatured (Comment)	Organism
apoprotein expressed in insect cells can be restored by addition of Cu2+, fully active	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
erythrocyte	Cu,Zn-SOD	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O2- + H+	-	Homo sapiens	O2 + H2O2	-	?
O2- + H+	-	Homo sapiens CuZn-SOD	O2 + H2O2	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
37	-	wild-type, purified stable for at least 1 week	Homo sapiens

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Release 2023.1 (January 2023)