General Stability | Organism |
---|---|
guanidinium chloride: 6 M, pH 7.5, 16 h, 23°C, stable | Thermus aquaticus |
urea: 8 M, pH 7.5, 16 h, 23°C, stable | Thermus aquaticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 2 atoms of manganese per molecule | Thermus aquaticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
4 * 21000, Mn-SOD, SDS-PAGE | Thermus aquaticus |
80000 | - |
Mn-SOD, gel filtration | Thermus aquaticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus aquaticus | - |
Mn-SOD | - |
Thermus aquaticus Mn-SOD | - |
Mn-SOD | - |
Purification (Comment) | Organism |
---|---|
Mn-SOD | Thermus aquaticus |
Renatured (Comment) | Organism |
---|---|
reconstitution of active enzyme after withdrawal of Mn2+ by addition of Mn2+ to apoprotein in 8 M urea at acid pH | Thermus aquaticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermus aquaticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O2- + H+ | - |
Thermus aquaticus | O2 + H2O2 | - |
? | |
O2- + H+ | - |
Thermus aquaticus Mn-SOD | O2 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 21000, Mn-SOD, SDS-PAGE | Thermus aquaticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
10 min, stable up to | Thermus aquaticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10.5 | 12 h, 23°C, stable | Thermus aquaticus |