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Literature summary for 1.14.99.4 extracted from

  • Rahim, M.A.; Sih, C.J.
    Mechanisms of steroid oxidation by microorganisms. XI. Enzymatic cleavage of the pregnane side chain (1966), J. Biol. Chem., 241, 3615-3623.
    View publication on PubMed
Search for more literature for 1.14.99.4

General Stability

General Stability Organism
1 mg/ml albumin stabilizes crude enzyme extract for 1 month when kept frozen Ilyonectria destructans
10 h dialysis of purified enzyme against 0.01 M Tris buffer, pH 7.5, 0.005 M EDTA, leads to 50% loss of activity Ilyonectria destructans
EDTA affords some protection, especially for crude extracts Ilyonectria destructans
preparation is highly unstable Ilyonectria destructans

Inhibitors

Inhibitors Comment Organism Structure
KCN
-
 Ilyonectria destructans
methylene blue
-
 Ilyonectria destructans
p-hydroxymercuribenzoate
-
 Ilyonectria destructans
phenazine methosulfate
-
 Ilyonectria destructans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
progesterone + NADPH + H+ + O2 Amorphotheca resinae
-
 Testosterone acetate + NADP+ + H2O
-
 ?
progesterone + NADPH + H+ + O2 Ilyonectria destructans
-
 Testosterone acetate + NADP+ + H2O
-
 ?
progesterone + NADPH + H+ + O2 Ilyonectria destructans ACC 11011
-
 Testosterone acetate + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Amorphotheca resinae
-
-
-
Ilyonectria destructans
-
 ACC 11011
-
Ilyonectria destructans ACC 11011
-
 ACC 11011
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ilyonectria destructans

Storage Stability

Storage Stability Organism
0-6°, 85% of the enzyme activity is lost in 24 h Ilyonectria destructans
0°C, 80% enzyme activity is lost in 7 days, half-life of a highly purified preparation is 12 h Ilyonectria destructans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains Ilyonectria destructans 16alpha-hydroxyandrost-4-ene-3,17-dione
-
 ?
16alpha,17alpha-oxidopregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains Ilyonectria destructans ACC 11011 16alpha-hydroxyandrost-4-ene-3,17-dione
-
 ?
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains, about 210% the rate of the reaction with progesterone Ilyonectria destructans androst-4-ene-3,17-dione + NADP+ + ?
-
 ?
17alpha-hydroxypregn-4-ene-3,20-dione + NADPH + O2 degradation of pregnane side chains, about 210% the rate of the reaction with progesterone Ilyonectria destructans ACC 11011 androst-4-ene-3,17-dione + NADP+ + ?
-
 ?
17alpha-methylpregn-4-ene-3,20-dione + NADPH + O2 about 4.3% the rate of the reaction with progesterone Ilyonectria destructans ?
-
 ?
deoxycorticosterone + NADPH + O2 degradation of pregnane side chains, about 64% the rate of the reaction with progesterone Ilyonectria destructans testosterone + NADP+ + ?
-
 ?
deoxycorticosterone + NADPH + O2 degradation of pregnane side chains, about 64% the rate of the reaction with progesterone Ilyonectria destructans ACC 11011 testosterone + NADP+ + ?
-
 ?
additional information cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates Ilyonectria destructans ?
-
 ?
additional information cortisol, 20alpha-hydroxy-16alpha,17alpha-oxidopregn-4-ene-3,20-dione and 20beta-hydroxypregn-4-ene-3-one are no substrates Ilyonectria destructans ACC 11011 ?
-
 ?
pregna-4,16-diene-3,20-dione + NADPH + O2 degradation of pregnane side chains, about 104% the rate of the reaction with progesterone Ilyonectria destructans androst-4-ene-3,17-dione
-
 ?
progesterone + NADPH + H+ + O2
-
 Amorphotheca resinae Testosterone acetate + NADP+ + H2O
-
 ?
progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans Testosterone acetate + NADP+ + H2O
-
 ?
progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans ACC 11011 Testosterone acetate + NADP+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 progesterone, deoxycorticosterone, 17alpha-hydroxypregn-4-ene-3,20-dione as substrates Ilyonectria destructans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.4 9 irreversible inactivated below Ilyonectria destructans
7 8 most stable Ilyonectria destructans

Cofactor

Cofactor Comment Organism Structure
NADPH absolute requirement, FMN, FAD, NADH or 2-amino-6,7-dimethyl-4-hydroxy-5,6,7,8-tetrahydropteridine cannot replace NADPH as hydrogen-donor Ilyonectria destructans