Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.29 extracted from

  • Park, J.; Dias, C.; Lee, S.; Valentini, S.; Sokabe, M.; Fraser, C.; Park, M.
    Production of active recombinant eIF5A: Reconstitution in E. coli of eukaryotic hypusine modification of eIF5A by its coexpression with modifying enzymes (2011), Protein Eng. Des. Sel., 24, 301-309.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.99.29

Cloned(Commentary)

Cloned (Comment) Organism
construction of a polycistronic vector encoding eIF5A, DHS and DOHH, expression in Escherichia coli strain BL21(DE3), overview. Effects of time and temperature on the expression of recombinant proteins and hypusine modification, DOHH effectively hydroxylates all the eIF5A(Dhp), a deoxyhypusine-containing intermediate, formed Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a polycistronic vector encoding eIF5A, DHS and DOHH, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2 Homo sapiens the DOHH reaction is irreversible eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
 ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2
-
 Homo sapiens eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
 ir
eIF5A-N6-(4-aminobutyl)-L-lysine + AH2 + O2 the DOHH reaction is irreversible Homo sapiens eIF5A-N6-(4-amino-2-hydroxybutyl)-L-lysine + A + H2O
-
 ir

Synonyms

Synonyms Comment Organism
deoxyhypusine hydroxylase
-
 Homo sapiens
DOHH
-
 Homo sapiens

General Information

General Information Comment Organism
evolution hypusine occurs only in eukaryotes and certain archaea, but not in eubacteria Homo sapiens
metabolism eukaryotic translation initiation factor 5A, eIF5A, is the only cellular protein that contains the polyamine-modified lysine, hypusine, i.e. N6-(4-amino-2-hydroxybutyl)lysine, which is formed post-translationally by two consecutive enzymatic reactions catalyzed by deoxyhypusine synthase, DHS, and deoxyhypusine hydroxylase, DOHH Homo sapiens
physiological function Hypusine modification is essential for the activity of eIF5A and for eukaryotic cell proliferation. eIF5A binds to the ribosome and stimulates translation in a hypusine-dependent manner Homo sapiens