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Literature summary for 1.14.99.24 extracted from

  • Wei, W.; Fan, S.; Wang, F.; Wei, D.
    A new steroid-transforming strain of Mycobacterium neoaurum and cloning of 3-ketosteroid 9alpha-hydroxylase in NwIB-01 (2010), Appl. Biochem. Biotechnol., 162, 1446-1456.
    View publication on PubMed

Application

Application Comment Organism
additional information strain NwIB-01 can be applied as excellent phytosterols-transformation strains in potential industrial applications Mycolicibacterium neoaurum

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree Mycolicibacterium neoaurum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45370
-
x * 45370, sequence calculation Mycolicibacterium neoaurum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mycolicibacterium neoaurum KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione ?
-
?
additional information Mycolicibacterium neoaurum NwIB-01 KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione ?
-
?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium neoaurum C9E9N6
-
-
Mycolicibacterium neoaurum NwIB-01 C9E9N6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione Mycolicibacterium neoaurum ?
-
?
additional information KSH cleaves the side chain specifically from soybean phytosterols to accumulate 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione Mycolicibacterium neoaurum NwIB-01 ?
-
?

Subunits

Subunits Comment Organism
? x * 45370, sequence calculation Mycolicibacterium neoaurum

Synonyms

Synonyms Comment Organism
3-ketosteroid 9alpha-hydroxylase
-
Mycolicibacterium neoaurum
KSH
-
Mycolicibacterium neoaurum

pI Value

Organism Comment pI Value Maximum pI Value
Mycolicibacterium neoaurum sequence calculation
-
5.46

General Information

General Information Comment Organism
metabolism key enzyme in steroid degradation. The two key enzymes, 3-ketosteroid DELTA1-dehydrogenase, KsdD, and 3-ketosteroid 9alpha-hydroxylase, KSH, are essential for the production and accumulation of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione from soy bean, Glycine max Mycolicibacterium neoaurum