General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Manduca sexta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00183 | - |
ecdysone | mitochondrial enzyme system | Manduca sexta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ecdysone + NADPH + O2 | Manduca sexta | serves as precursor of ecdysterone, the active moulting hormone in larval insects | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | Manduca sexta | alpha-ecdysone, a secretory product of prothoracic glands | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | Manduca sexta | last step in biosynthesis of insect molting hormone | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
tobacco hornworm | - |
Purification (Comment) | Organism |
---|---|
- |
Manduca sexta |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein | Manduca sexta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000466 | - |
mitochondrial enzyme system | Manduca sexta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-ecdysone + NADPH + O2 | - |
Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | serves as precursor of ecdysterone, the active moulting hormone in larval insects | Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | alpha-ecdysone, a secretory product of prothoracic glands | Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
ecdysone + NADPH + O2 | last step in biosynthesis of insect molting hormone | Manduca sexta | 20-hydroxyecdysone + NADP+ + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | essential | Manduca sexta | |
NADPH | - |
Manduca sexta |