Literature summary for 1.14.99.15 extracted from

Bernhardt, F.H.; Nastainczyk, W.; Seydewitz, U.
Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida (1977), Eur. J. Biochem., 72, 107-115.

Search for more literature for 1.14.99.15

Activating Compound

Activating Compound	Comment	Organism	Structure
Putidamonooxin	-	Pseudomonas putida

Inhibitors

Inhibitors	Comment	Organism	Structure
4-(2-Pyridylazo)resorcinol	-	Pseudomonas putida
4-tert-Butylbenzoate	competitive inhibitor of the O-demethylation of 3-nitro-4-methoxybenzoate, hinders O2-binding or O2-activation	Pseudomonas putida
8-hydroxyquinoline	-	Pseudomonas putida
Amytal	-	Pseudomonas putida
Atebrin	-	Pseudomonas putida
Bathocuproinedisulfonate	-	Pseudomonas putida
bathophenanthrolinedisulfate	preferentially inhibiting putidamonooxin	Pseudomonas putida
Cd2+	-	Pseudomonas putida
Co2+	-	Pseudomonas putida
Cu2+	-	Pseudomonas putida
Cumylhydroperoxide	-	Pseudomonas putida
Hg2+	-	Pseudomonas putida
Iodosobenzene	-	Pseudomonas putida
Ni2+	-	Pseudomonas putida
p-chloromercuribenzoate	-	Pseudomonas putida
rotenone	-	Pseudomonas putida
Zn2+	-	Pseudomonas putida

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00007	-	4-Methoxybenzoate	-	Pseudomonas putida
0.0003	-	3-Nitro-4-methoxybenzoate	-	Pseudomonas putida
0.00063	-	NADH	reconstituted enzyme	Pseudomonas putida
0.0058	-	4-methylaminobenzoate	-	Pseudomonas putida
0.009	-	4-Methylbenzoate	-	Pseudomonas putida
0.024	-	4-trifluoromethylbenzoate	-	Pseudomonas putida
0.029	-	4-hydroxybenzoate	-	Pseudomonas putida
0.055	-	O2	+ benzoate	Pseudomonas putida
0.14	-	NADPH	reconstituted enzyme	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	both enzyme components are iron-sulfur proteins	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-methoxybenzoate + NADH + O2	Pseudomonas putida	-	4-hydroxybenzoate + NAD+ + H2O + formaldehyde	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.018	-	superoxide anion instead of substrate, putidamonooxin	Pseudomonas putida
0.192	-	3-nitro-4-methoxybenzoate, putidamonooxin	Pseudomonas putida
0.27	-	4-methoxybenzoate, putidamonooxin	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-nitro-4-methoxybenzoate + NADH + O2	-	Pseudomonas putida	3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde	-	?
3-nitro-4-methoxybenzoate + NADPH + O2	-	Pseudomonas putida	3-nitro-4-hydroxybenzoate + NADP+ + H2O + formaldehyde	-	r
4-hydroxybenzoate + NADH + O2	-	Pseudomonas putida	3,4-dihydroxybenzoate + NAD+ + H2O	ring hydroxylation	?
4-methoxybenzoate + NADH + O2	-	Pseudomonas putida	4-hydroxybenzoate + NAD+ + H2O + formaldehyde	-	?
4-methylbenzoate + NADH + O2	-	Pseudomonas putida	4-carboxybenzylalcohol + NAD+ + H2O	-	?
4-trifluoromethylbenzoate + NADH + O2	-	Pseudomonas putida	?	-	?
N-methyl-4-aminobenzoate + NADH + O2	-	Pseudomonas putida	4-aminobenzoate + NAD+ + H2O + formaldehyde	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Pseudomonas putida
NADH	requirement, can be replaced by NADPH with 40% efficiency	Pseudomonas putida
NADPH	can replace NADH with 40% efficiency	Pseudomonas putida

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Release 2023.1 (January 2023)