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Literature summary for 1.14.99.15 extracted from

  • Wende, P.; Pfleger, K.; Bernhardt, F.H.
    Dioxygen activation by putidamonooxin: substrate-modulated reaction of activated dioxygen (1982), Biochem. Biophys. Res. Commun., 140, 527-532.
No PubMed abstract available
Search for more literature for 1.14.99.15

Activating Compound

Activating Compound Comment Organism Structure
Putidamonooxin
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ both enzyme components are iron-sulfur proteins Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-methoxybenzoate + NADH + O2 Pseudomonas putida
-
 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Reaction

Reaction Comment Organism Reaction ID
4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O the bacterial enzyme is a two-component enzyme, consisting of an iron-sulfur flavoprotein (FMN), NADH-putidamonooxin-reductase and a ferredoxin-type, oxygen-activating protein, putidamonooxin Pseudomonas putida
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxybenzoate + NADH + O2 partial uncoupler Pseudomonas putida 3,4-dihydroxybenzoate + NAD+ + H2O in the uncoupled part of the reaction, 3-hydroxybenzoate is not hydroxylated and H2O2 is a product, too ?
3-nitro-4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 3-nitro-4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
4-hydroxybenzoate + NADH + O2
-
 Pseudomonas putida 3,4-dihydroxybenzoate + NAD+ + H2O
-
 ?
4-methoxybenzoate + NADH + O2
-
 Pseudomonas putida 4-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
 ?
4-vinylbenzoate + NADH + O2 external dioxygenase reaction by substrate induced modulation Pseudomonas putida 4-glycylbenzoate + NAD+ + H2O
-
 ?
additional information overview: substrates that are not oxygenized while NADH-oxidation and O2-consumption are catalyzed, such as: benzoate, 3-chlorobenzoate, 4-chlorobenzoate, 2-hydroxybenzoate, 4-bromobenzoate, 2-aminobenzoate, 3-aminobenzoate, 4-trifluoromethylbenzoate, 4-tert-butylbenzoate Pseudomonas putida additional information uncoupling substrates are not oxygenized, NAD+ and H2O2 being the only products of the reaction ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Pseudomonas putida