Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.99.14 extracted from

  • Jayanthi, C.R.; Madyastha, P.; Madyastha, K.M.
    Microsomal 11alpha-hydroxylation of progesterone in Aspergillus ochraceus: Part I: Characterization of the hydroxylase system (1982), Biochem. Biophys. Res. Commun., 106, 1262-1268.
    View publication on PubMed
Search for more literature for 1.14.99.14

Inhibitors

Inhibitors Comment Organism Structure
carbon monoxide
-
 Aspergillus ochraceus
cytochrome c
-
 Aspergillus ochraceus
Metyrapone
-
 Aspergillus ochraceus
Metyrapone
-
 Rhizopus stolonifer
N-methyl maleimide
-
 Aspergillus ochraceus
p-chloromercuribenzoate
-
 Aspergillus ochraceus
SKF-525A
-
 Aspergillus ochraceus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Aspergillus ochraceus 5829
-
cytosol
-
 Rhizopus stolonifer 5829
-
microsome
-
 Aspergillus ochraceus
-
-
microsome
-
 Rhizopus stolonifer
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
progesterone + NADPH + O2 Rhizopus arrhizus
-
 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 Rhizopus stolonifer
-
 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 Aspergillus ochraceus activity dependent upon NADPH and O2 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 Aspergillus ochraceus NRRL 405 activity dependent upon NADPH and O2 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 Aspergillus ochraceus TS activity dependent upon NADPH and O2 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus ochraceus
-
-
-
Aspergillus ochraceus NRRL 405
-
-
-
Aspergillus ochraceus TS
-
-
-
Rhizopus arrhizus
-
-
-
Rhizopus stolonifer
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus ochraceus
-
mycelium
-
 Rhizopus stolonifer
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0484
-
 cytosol Aspergillus ochraceus
0.388
-
 microsomes Aspergillus ochraceus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
progesterone + NADPH + O2
-
 Rhizopus arrhizus 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2
-
 Aspergillus ochraceus 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2
-
 Rhizopus stolonifer 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 activity dependent upon NADPH and O2 Aspergillus ochraceus 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2
-
 Aspergillus ochraceus NRRL 405 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 activity dependent upon NADPH and O2 Aspergillus ochraceus NRRL 405 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2
-
 Aspergillus ochraceus TS 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?
progesterone + NADPH + O2 activity dependent upon NADPH and O2 Aspergillus ochraceus TS 11alpha-hydroxyprogesterone + NADP+ + H2O
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
 Aspergillus ochraceus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Rhizopus arrhizus
cytochrome P450
-
 Aspergillus ochraceus
cytochrome P450
-
 Rhizopus stolonifer
NADH NADH is far less efficient than NADPH Aspergillus ochraceus
NADPH
-
 Aspergillus ochraceus