Cloned (Comment) | Organism |
---|---|
functional expression of C-terminally or N-terminally His6-tagged and non-tagged PGHS-2 in Pichia pastoris strain GS115, GS117 and KM71 using native or yeast signal sequences, method evaluation by enzyme activity, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane associated glycoprotein | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + AH2 + 2 O2 | Homo sapiens | - |
prostaglandin H2 + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P23219 | - |
- |
Homo sapiens | P35354 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | membrane associated glycoprotein | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally or N-terminally His6-tagged PGHS-2 from Pichia pastoris strain GS115 microsomes by ultracentrifugation, nickel affinity and anion exchange chromatography, purification of the C-terminally His6 tagged hPGHS-2 is more efficient, relative efficiency of the detergents used for solubilization of the recombinant hPGHS-2, CHAPS is most effective, method evaluation, overview | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4220 | - |
purified recombinant His6-tagged PGHS-2, pH 8.0, 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + AH2 + 2 O2 | - |
Homo sapiens | prostaglandin H2 + A + H2O | - |
? | |
arachidonate + reduced N,N,N',N'-tetramethylphenylenediamine + 2 O2 | - |
Homo sapiens | prostaglandin H2 + oxidized N,N,N',N'-tetramethylphenylenediamine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGHS-1 | - |
Homo sapiens |
PGHS-2 | - |
Homo sapiens |
prostaglandin H synthase-1 | - |
Homo sapiens |
prostaglandin H synthase-2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | peroxidase activity of PGHS requires reducing co-substrates as electron donors | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | prostaglandin H synthase-1 catalyzes the first two steps in prostaglandin synthesis | Homo sapiens |
metabolism | prostaglandin H synthase-2 catalyzes the first two steps in prostaglandin synthesis | Homo sapiens |