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Literature summary for 1.14.99.1 extracted from

  • Deeb, R.S.; Hao, G.; Gross, S.S.; Laine, M.; Qiu, J.H.; Resnick, B.; Barbar, E.J.; Hajjar, D.P.; Upmacis, R.K.
    Heme catalyzes tyrosine 385 nitration and inactivation of prostaglandin H2 synthase-1 by peroxynitrite (2006), J. Lipid Res., 47, 898-911.
    View publication on PubMed
Search for more literature for 1.14.99.1

Activating Compound

Activating Compound Comment Organism Structure
peroxynitrite peroxidase activity, MS-analysis reveals that tyrosine 385 is a target for nitration by ONOO- only when heme is present Ovis aries

Cloned(Commentary)

Cloned (Comment) Organism
-
 Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
aspirin cyclooxygenase activity Ovis aries

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Ovis aries
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 confirmed by SDS-PAGE, Western blot analysis and MALDI-TOF Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonate + AH2 + O2 Ovis aries
-
 prostaglandin H2 + A + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
N-octyl-beta-D-glucopyranoside, Tween-20, CHAPS, and maltoside are used for solubilization of prostaglandin H2 synthase-1, frozen microsomal extracts are thawed and purified using a fast-flow DEAE-Sepharose and a S-300 column Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
seminal vesicle
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonate + AH2 + O2
-
 Ovis aries prostaglandin H2 + A + H2O
-
 ?

Synonyms

Synonyms Comment Organism
PGHS-1
-
 Ovis aries
prostaglandin H2 synthase-1
-
 Ovis aries

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 activity assay Ovis aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Ovis aries

Cofactor

Cofactor Comment Organism Structure
heme hemin and hematin Ovis aries