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Literature summary for 1.14.20.1 extracted from

  • Lloyd, M.D.; Lee, H.J.; Harlos, K.; Zhang, Z.H.; Baldwin, J.E.; Schofield, C.J.; Charnock, J.M.; Garner, C.D.; Hara, T.; Terwisscha van Scheltinga, A.C.; Valeg.Ang.rd, K.; Viklund, J.A.C.; Hajdu, J.; Andersson, I.; Danielsson, A.; Bhikhabhai, R.
    Studies on the active site of deacetoxycephalosporin C synthase (1999), J. Mol. Biol., 287, 943-960.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces clavuligerus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop method, recombinant enzyme expressed in Escherichia coli Streptomyces clavuligerus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
Penicillin N
-
Streptomyces clavuligerus
1.3
-
adipyl 6-aminopenicillanic acid
-
Streptomyces clavuligerus
2.03
-
penicillin G
-
Streptomyces clavuligerus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Streptomyces clavuligerus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34554
-
x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry Streptomyces clavuligerus

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus P18548
-
-

Purification (Commentary)

Purification (Comment) Organism
efficient large-scale purification Streptomyces clavuligerus
recombinant enzyme expressed in Escherichia coli Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66
-
recombinant enzyme Streptomyces clavuligerus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adipyl-6-aminopenicillanic acid + 2-oxoglutarate + O2 poor substrate Streptomyces clavuligerus 7-adipylaminodesacetoxycephalosporanic acid + succinate + CO2 + H2O
-
?
penicillin G + 2-oxoglutarate + O2
-
Streptomyces clavuligerus phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O
-
?
penicillin N + 2-oxoglutarate + O2
-
Streptomyces clavuligerus deacetoxycephalosporin C + succinate + CO2 + H2O
-
?

Subunits

Subunits Comment Organism
? x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry Streptomyces clavuligerus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.062
-
penicillin G
-
Streptomyces clavuligerus
0.073
-
adipyl 6-aminopenicillanic acid
-
Streptomyces clavuligerus
0.42
-
Penicillin N
-
Streptomyces clavuligerus