Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces clavuligerus |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, recombinant enzyme expressed in Escherichia coli | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
Penicillin N | - |
Streptomyces clavuligerus | |
1.3 | - |
adipyl 6-aminopenicillanic acid | - |
Streptomyces clavuligerus | |
2.03 | - |
penicillin G | - |
Streptomyces clavuligerus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Streptomyces clavuligerus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34554 | - |
x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | P18548 | - |
- |
Purification (Comment) | Organism |
---|---|
efficient large-scale purification | Streptomyces clavuligerus |
recombinant enzyme expressed in Escherichia coli | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
66 | - |
recombinant enzyme | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-6-aminopenicillanic acid + 2-oxoglutarate + O2 | poor substrate | Streptomyces clavuligerus | 7-adipylaminodesacetoxycephalosporanic acid + succinate + CO2 + H2O | - |
? | |
penicillin G + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | phenylacetyl-7-aminodeacetoxy cephalosporanic acid + succinate + CO2 + H2O | - |
? | |
penicillin N + 2-oxoglutarate + O2 | - |
Streptomyces clavuligerus | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34554, in solution the monomeric apoenzyme is in equilibrium with atrimeric form, electrospray mass spectrometry | Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
penicillin G | - |
Streptomyces clavuligerus | |
0.073 | - |
adipyl 6-aminopenicillanic acid | - |
Streptomyces clavuligerus | |
0.42 | - |
Penicillin N | - |
Streptomyces clavuligerus |