Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.9 extracted from

  • Lang, A.; Polnick, S.; Nicke, T.; William, P.; Patallo, E.; Naismith, J.; Van Pee, K.
    Changing the regioselectivity of the tryptophan 7-halogenase PrnA by site-directed mutagenesis (2011), Angew. Chem. Int. Ed. Engl., 50, 2951-2953.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
F103A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Pseudomonas fluorescens
F103A/W455A inactive Pseudomonas fluorescens
H101A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Pseudomonas fluorescens
W455A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme Pseudomonas fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0499
-
tryptophan His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication Pseudomonas fluorescens
0.1986
-
tryptophan His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication Pseudomonas fluorescens
1.785
-
tryptophan His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication Pseudomonas fluorescens
1.814
-
tryptophan His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tryptophan + FADH2 + Cl- + O2 + H+ Pseudomonas fluorescens
-
7-chloro-L-tryptophan + FAD + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel chelating Sepharose column chromatography Pseudomonas fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tryptophan + FADH2 + Cl- + O2 + H+
-
Pseudomonas fluorescens 7-chloro-L-tryptophan + FAD + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
PrnA
-
Pseudomonas fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
tryptophan His-tagged recombinant mutant enzyme W455A, at 30°C, pH not specified in the publication Pseudomonas fluorescens
0.021
-
tryptophan His-tagged recombinant mutant enzyme H101A, at 30°C, pH not specified in the publication Pseudomonas fluorescens
0.067
-
tryptophan His-tagged recombinant mutant enzyme F103A, at 30°C, pH not specified in the publication Pseudomonas fluorescens
0.11
-
tryptophan His-tagged recombinant wild type enzyme, at 30°C, pH not specified in the publication Pseudomonas fluorescens

Cofactor

Cofactor Comment Organism Structure
flavin dependent on Pseudomonas fluorescens