Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.19.9 extracted from

  • Yeh, E.; Cole, L.J.; Barr, E.W.; Bollinger, J.M.; Ballou, D.P.; Walsh, C.T.
    Flavin redox chemistry precedes substrate chlorination during the reaction of the flavin-dependent halogenase RebH (2006), Biochemistry, 45, 7904-7912.
    View publication on PubMed
Search for more literature for 1.14.19.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Lentzea aerocolonigenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information stopped-flow analysis of flavin intermediates Lentzea aerocolonigenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lentzea aerocolonigenes first step in biosynthesis of rebeccamycin ?
-
 ?
tryptophan + FADH2 + O2 + Cl- + H+ Lentzea aerocolonigenes initial step of rebeccamycin biosynthesis 7-chlorotryptophan + FAD + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Lentzea aerocolonigenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
RebH is purified by size-exclusion chromatography Lentzea aerocolonigenes

Reaction

Reaction Comment Organism Reaction ID
tryptophan + FADH2 + chloride + O2 + H+ = 7-chloro-L-tryptophan + FAD + 2 H2O FADH2 and O2 form an 4alpha-FAD-OOH intermediate that decays to 4alpha-FAD-OH. Tryptophan likely does not react directly with any flavin intermediate. Substrate chlorination occurs after completion of the flavin redox reactions Lentzea aerocolonigenes
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information first step in biosynthesis of rebeccamycin Lentzea aerocolonigenes ?
-
 ?
tryptophan + FADH2 + O2 + Cl- + H+ initial step of rebeccamycin biosynthesis Lentzea aerocolonigenes 7-chlorotryptophan + FAD + H2O
-
 ?

Synonyms

Synonyms Comment Organism
halogenase
-
 Lentzea aerocolonigenes
RebH
-
 Lentzea aerocolonigenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 end-point assay for single-turnover reactions Lentzea aerocolonigenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 FADH2 4°C Lentzea aerocolonigenes
0.05
-
 tryptophan ranges from 0.02 s-1 to 0.05 s-1 for different preparations, 25°C Lentzea aerocolonigenes
0.12
-
 FADH2 25°C Lentzea aerocolonigenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 end-point assay for single-turnover reactions Lentzea aerocolonigenes

Cofactor

Cofactor Comment Organism Structure
FADH2
-
 Lentzea aerocolonigenes