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Literature summary for 1.14.19.6 extracted from

  • Amiri, R.M.; Yureva, N.O.; Shimshilashvili, K.R.; Goldenkova-Pavlova, I.V.; Pchelkin, V.P.; Kuznitsova, E.I.; Tsydendambaev, V.D.; Trunova, T.I.; Los, D.A.; Jouzani, G.S.; Nosov, A.M.
    Expression of acyl-lipid Delta12-desaturase gene in prokaryotic and eukaryotic cells and its effect on cold stress tolerance of potato (2010), J. Integr. Plant Biol., 52, 289-297.
    View publication on PubMed
Search for more literature for 1.14.19.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum cells Synechocystis sp.
gene desA, recombinant expression of the desA-licBM3 hybrid in transgenic Escherichia coli strains XL1Blue and BL21 and Solanum tuberosum plants Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of a hybrid of desA and reporter gene encoding thermostable lichenase, licBM3. The desaturase can enhance tolerance to cold stress in potato, and desaturase and lichenase retain their functionality in the structure of the hybrid protein where the enzymatic activity of target gene product is higher than in the case of reporter lichenase gene absence in the construction, phenotypes, overview Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40500
-
 x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE Synechocystis sp.
66000
-
 x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE Synechocystis sp.
66000
-
 x * 66000, DesA As hybrid with lichenase LicBM3, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Synechocystis sp.
-
 1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 gene desA
-
Synechocystis sp. P20388
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-oleoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Synechocystis sp. 1-linoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 40500, DesA, SDS-PAGE, x * 66000, about, recombinant DesA-LicBM3 hybrid protein, SDS-PAGE Synechocystis sp.
? x * 66000, DesA As hybrid with lichenase LicBM3, SDS-PAGE Synechocystis sp.

Synonyms

Synonyms Comment Organism
acyl-lipid DELTA12-desaturase
-
 Synechocystis sp.
DesA
-
 Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
 Synechocystis sp.

General Information

General Information Comment Organism
additional information expression profile of desA gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic, Escherichia coli and eukaryotic, Solanum tuberosum cells, overview Synechocystis sp.