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Literature summary for 1.14.19.6 extracted from
- Cloning and molecular characterization of DELTA12-fatty acid desaturase gene from Mortierella isabellina (2006), World J. Gastroenterol., 12, 3373-3379.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of DELTA12-fatty acid desaturase genes in Escherichia coli strain BL21 and Saccharomyces cerevisiae strain IN-VSc1 leads to production of an active enzyme which converts 17.876% and 17.604% of oleic acid to linoleic acid, GC-MS detection in vitro and in vivo | Umbelopsis isabellina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
SDS-PAGE of cell membranes of transformed Escherichia coli | Umbelopsis isabellina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Umbelopsis isabellina | P59668 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal condition for expressing DELTA12-fatty acid desaturase in Saccharomyces cerevisiae is 3% galactose induction for 24 h at 15°C | Umbelopsis isabellina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oleic acid + reduced acceptor + O2 | - |
Umbelopsis isabellina | linoleic acid + acceptor + H2O | - |
? |  |
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