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Literature summary for 1.14.19.36 extracted from

  • Chintalapati, S.; Prakash, J.S.; Singh, A.K.; Ohtani, S.; Suzuki, I.; Murata, N.; Shivaji, S.
    Desaturase genes in a psychrotolerant Nostoc sp. are constitutively expressed at low temperature (2007), Biochem. Biophys. Res. Commun., 362, 81-87.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene desB, DNA and amino acid sequence determination and analysis, phylogenetic tree, genes desA, desB, and desC are in tandem and have an ORF of 822 bp, 1053 bp, and 1080 bp, respectively, and are separated by intergenic regions, the three genes do not form an operon, RT-PCR enzyme expression analysis Nostoc sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane transmembrane domains at residues 43-65, 71-93, 200-222, and 231-253 Nostoc sp. 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41800
-
x * 41800, about, sequence calculation Nostoc sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Nostoc sp.
-
a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Nostoc sp.
-
a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Nostoc sp. Q704F0 gene desB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a 1-gamma-linolenoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
Nostoc sp. a 1-stearidonoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?
a 1-linoleoyl-2-acyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
Nostoc sp. a 1-alpha-linolenoyl-2-acyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
?

Subunits

Subunits Comment Organism
? x * 41800, about, sequence calculation Nostoc sp.

Synonyms

Synonyms Comment Organism
DesB
-
Nostoc sp.
Omega 3 acyl-lipid desaturase
-
Nostoc sp.

Cofactor

Cofactor Comment Organism Structure
Ferredoxin
-
Nostoc sp.

Expression

Organism Comment Expression
Nostoc sp. antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids, but constitutive expression of desB gene when cultures grown at 25°C are shifted to 10°C up

General Information

General Information Comment Organism
metabolism the conversion of the C18 fatty acid to C18:3(9,12,15) is catalyzed in a step wise manner by DesC, DesA, and DesB, respectively Nostoc sp.
additional information antarctic psychrotolerant, Nostoc sp. SO-36, when grown at 25°C and then shifted to 10°C, shows an increase in the tri-unsaturated fatty acid [C18:3(9,12,15)] at the expense of mono- [C18:1(9)] and di-unsaturated [C18:2(9,12)] fatty acids Nostoc sp.